A novel bistable device to study mechanosensitive cell responses to instantaneous stretch
| العنوان: | A novel bistable device to study mechanosensitive cell responses to instantaneous stretch |
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| المؤلفون: | Young Choi, Giulia Morlino, Amparo Toboso-Navasa, Raoul Hopf, Francesca Michela Pramotton, Anne Bigot, Andrea Taddei, Nikola Cesarovic, Volkmar Falk, Edoardo Mazza, Costanza Giampietro |
| المصدر: | Biomaterials Advances, 141 |
| بيانات النشر: | ETH Zurich, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Biomaterials, Uniaxial stretch, Mechanotransduction, Instantaneous stretch, Biomedical Engineering, Bioengineering, Bistable device |
| الوصف: | The behavior of cells and tissues in vivo is determined by the integration of multiple biochemical and mechanical signals. Of the mechanical signals, stretch has been studied for decades and shown to contribute to pathophysiological processes. Several different stretch devices have been developed for in vitro investigations of cell stretch. In this work, we describe a new 3D-printed uniaxial stretching device for studying cell response to rapid deformation. The device is a bistable compliant mechanism holding two equilibrium states—an unstretched and stretched configuration—without the need of an external actuator. Furthermore, it allows multiple simultaneous measurements of different levels of stretch on a single substrate and is compatible with standard immunofluorescence imaging of fixed cells as well as live-cell imaging. To demonstrate the effectiveness of the device to stretch cells, a test case using aligned myotubes is presented. Leveraging material area changes associated with deformation of the substrate, changes in nuclei density provided evidence of affine deformation between cells and substrate. Furthermore, intranuclear deformations were also assessed and shown to deform non-affinely. As a proof-of-principle of the use of the device for mechanobiological studies, we uniaxially stretched aligned healthy and dystrophic myotubes that displayed different passive mechanical responses, consistent with previous literature in the field. We also identified a new feature in the mechanoresponse of dystrophic myotubes, which is of potential interest for identifying the diseased cells based on a quick mechanical readout. While some applications of the device for elucidating passive mechanical responses are demonstrated, the simplicity of the device allows it to be potentially used for other modes of deformation with little modifications. Biomaterials Advances, 141 ISSN:2772-9508 |
| وصف الملف: | application/application/pdf |
| اللغة: | English |
| تدمد: | 2772-9508 |
| DOI: | 10.3929/ethz-b-000575673 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f59d74135bdde03131bbeaf315fde390 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f59d74135bdde03131bbeaf315fde390 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 27729508 |
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| DOI: | 10.3929/ethz-b-000575673 |