Evaluation of hazardousness of refractory fibers by cell magnetometry
| العنوان: | Evaluation of hazardousness of refractory fibers by cell magnetometry |
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| المؤلفون: | Yoshiharu Aizawa, Makoto Kotani, Yuichiro Kudo |
| المصدر: | The Journal of Toxicological Sciences. 35:807-815 |
| بيانات النشر: | Japanese Society of Toxicology, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Cell Survival, Scanning electron microscope, Phagocytosis, Cell, Cell Culture Techniques, Analytical chemistry, Toxicology, Cell Line, Magnetics, Mice, chemistry.chemical_compound, Microscopy, Electron, Transmission, Lactate dehydrogenase, Toxicity Tests, medicine, Animals, Cytotoxic T cell, Cytotoxicity, Lactate Dehydrogenases, Mineral Fibers, Equipment Design, Phosphate, Ferrosoferric Oxide, medicine.anatomical_structure, chemistry, Cell culture, Macrophages, Peritoneal, Microscopy, Electron, Scanning, Nuclear chemistry |
| الوصف: | This study was carried out to assess the cytotoxicity of three kinds of refractory fibers (RFs), RF1, RF2, and RF3, by cell magnetometry, lactate dehydrogenase (LDH) assay and morphological observation by scanning electron microscopy, using a mouse-derived cultured cell line, RAW264.7. As an indicator for cell magnetometry, Fe(3)O(4) was added to RAW264.7 cells. RF1, RF2 and RF3 were each added to an aliquot of this solution to make final concentrations of 250, 500 and 1,000 µg/ml in the experimental group. Phosphate buffered solution was added to make the control solution (n = 6). After culturing for 48 hr, the solution was magnetized from outside using a cell magnetometric apparatus, and the remnant magnetic field was measured for 20 min postmagnetization. In cell magnetometry, a significant delay of relaxation compared to that of the control was observed. In the LDH assay, LDH release into the culture medium was observed by addition of RFs. Furthermore, a quantity-dependent relationship was found between the quantity of RF added and the cytotoxicity in cell magnetometry and LDH assay. Morphological examination revealed incomplete phagocytosis of fibers and a decrease of microvilli in the experimental groups. These results suggest that RFs are cytotoxic to RAW264.7 cells, showing concentration-dependent cytotoxicity, and have a possible risk of cytotoxicity similar to that of asbestos. Further studies by pulmonary magnetometry are necessary to assess the hazardousness of RFs. |
| تدمد: | 1880-3989 0388-1350 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5df135ad7ee5af788f91b8158dfe21c https://doi.org/10.2131/jts.35.807 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f5df135ad7ee5af788f91b8158dfe21c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18803989 03881350 |
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