Evidence that DNA polymerase δ contributes to initiating leading strand DNA replication in Saccharomyces cerevisiae
| العنوان: | Evidence that DNA polymerase δ contributes to initiating leading strand DNA replication in Saccharomyces cerevisiae |
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| المؤلفون: | James E. Haber, Zhi-Xiong Zhou, Qiuqin Wu, Thomas A. Kunkel, Adam B. Burkholder, Phillip B. Cox, Marta A. Garbacz, Scott A. Lujan |
| المصدر: | Nature Communications Nature Communications, Vol 9, Iss 1, Pp 1-11 (2018) |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, DNA Replication, DNA polymerase, Science, Saccharomyces cerevisiae, General Physics and Astronomy, Replication Origin, Origin of replication, medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, 0302 clinical medicine, medicine, lcsh:Science, Polymerase, DNA Polymerase III, Mutation, Multidisciplinary, biology, Models, Genetic, DNA replication, General Chemistry, DNA Polymerase II, biology.organism_classification, Nuclear DNA, Cell biology, 030104 developmental biology, Phenotype, biology.protein, Proofreading, lcsh:Q, 030217 neurology & neurosurgery |
| الوصف: | To investigate nuclear DNA replication enzymology in vivo, we have studied Saccharomyces cerevisiae strains containing a pol2-16 mutation that inactivates the catalytic activities of DNA polymerase ε (Pol ε). Although pol2-16 mutants survive, they present very tiny spore colonies, increased doubling time, larger than normal cells, aberrant nuclei, and rapid acquisition of suppressor mutations. These phenotypes reveal a severe growth defect that is distinct from that of strains that lack only Pol ε proofreading (pol2-4), consistent with the idea that Pol ε is the major leading-strand polymerase used for unstressed DNA replication. Ribonucleotides are incorporated into the pol2-16 genome in patterns consistent with leading-strand replication by Pol δ when Pol ε is absent. More importantly, ribonucleotide distributions at replication origins suggest that in strains encoding all three replicases, Pol δ contributes to initiation of leading-strand replication. We describe two possible models. DNA polymerases δ and ε (Pols δ and ε) are thought to be responsible for lagging and leading strand synthesis, respectively. Here the authors present evidence that Pol δ contributes to the initiation of leading strand replication in budding yeast by synthesizing DNA of both strands at replication origins. |
| تدمد: | 2041-1723 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f61f047d08ba22366e03b563635477ce https://pubmed.ncbi.nlm.nih.gov/29487291 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f61f047d08ba22366e03b563635477ce |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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