Retrograde Fluxes of Focal Adhesion Proteins in Response to Cell Migration and Mechanical Signals
| العنوان: | Retrograde Fluxes of Focal Adhesion Proteins in Response to Cell Migration and Mechanical Signals |
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| المؤلفون: | Yu-li Wang, Wei-hui Guo |
| المصدر: | Molecular Biology of the Cell. 18:4519-4527 |
| بيانات النشر: | American Society for Cell Biology (ASCB), 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Focal Adhesions, Role of cell adhesions in neural development, PTK2, Cell migration, Articles, Cell Biology, Protein-Tyrosine Kinases, Biology, Actin cytoskeleton, Actins, Zyxin, Cell biology, Focal adhesion, Extracellular matrix, Mice, Cell Movement, Metalloproteins, NIH 3T3 Cells, Animals, Phosphotyrosine, Molecular Biology, Actin, Signal Transduction |
| الوصف: | Recent studies suggest that mechanical signals mediated by the extracellular matrix play an essential role in various physiological and pathological processes; yet, how cells respond to mechanical stimuli remains elusive. Using live cell fluorescence imaging, we found that actin filaments, in association with a number of focal adhesion proteins, including zyxin and vasodilator-stimulated phosphoprotein, undergo retrograde fluxes at focal adhesions in the lamella region. This flux is inversely related to cell migration, such that it is amplified in fibroblasts immobilized on micropatterned islands. In addition, the flux is regulated by mechanical signals, including stretching forces applied to flexible substrates and substrate stiffness. Conditions favoring the flux share the common feature of causing large retrograde displacements of the interior actin cytoskeleton relative to the substrate anchorage site, which may function as a switch translating mechanical input into chemical signals, such as tyrosine phosphorylation. In turn, the stimulation of actin flux at focal adhesions may function as part of a feedback mechanism, regulating structural assembly and force production in relation to cell migration and mechanical load. The retrograde transport of associated focal adhesion proteins may play additional roles in delivering signals from focal adhesions to the interior of the cell. |
| تدمد: | 1939-4586 1059-1524 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6360a7e18f4cc9c588db3391186c90b https://doi.org/10.1091/mbc.e07-06-0582 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f6360a7e18f4cc9c588db3391186c90b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19394586 10591524 |
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