Innate immunity mediated by dendritic cells/macrophages plays a central role in the early period in tumor treatment using gene of Mycobacterium tuberculosis antigen
| العنوان: | Innate immunity mediated by dendritic cells/macrophages plays a central role in the early period in tumor treatment using gene of Mycobacterium tuberculosis antigen |
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| المؤلفون: | Yoshiyuki Koyama, Tomoko Ito, Ryoji Kanegi, Kazuyuki Uchida, Toshio Inaba, Kikuya Sugiura, James K. Chambers, Aya Hasegawa, Kenichi Watanabe, Takahiro Ushigusa, Shingo Hatoya |
| المصدر: | The Journal of Veterinary Medical Science |
| بيانات النشر: | Japanese Society of Veterinary Science, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, tumor, early secretory antigenic target-6, Genetic enhancement, Immunology, Population, Melanoma, Experimental, Biology, Transfection, Mice, 03 medical and health sciences, 0302 clinical medicine, Immune system, Bacterial Proteins, Antigen, Animals, education, innate immunity, Antigens, Bacterial, education.field_of_study, Innate immune system, Full Paper, General Veterinary, Macrophages, Dendritic Cells, Neoplasms, Experimental, Acquired immune system, gene therapy, Immunity, Innate, Mice, Inbred C57BL, 030104 developmental biology, 030220 oncology & carcinogenesis, Cancer research, dendritic cells/macrophages, Tumor necrosis factor alpha, Neoplasm Transplantation |
| الوصف: | By using a complex of DNA, polyethylenimine and chondroitin sulfate, the in vivo transfection of early secretory antigenic target-6 (ESAT-6) gene into tumor cells was found to cause significant suppression of the tumor growth. In order to apply the method in clinical cancer treatment in dogs and cats, mechanisms underlying the suppressive effects were investigated in a tumor-bearing mouse model. The transfection efficiency was only about 10%, but the transfection of ESAT-6 DNA nevertheless induced systemic immune responses against ESAT-6. By triple injection of ESAT-6 DNA at three day intervals, the tumor was significantly reduced and almost disappeared by 5 days after the start of treatment, and did not increase for more than 15 days after the final treatment. In the immunohistochemistry, a larger number of dendritic cells (DCs)/macrophages expressing ionized calcium-binding adapter molecule 1 and CD3+ T cells was observed in tumors treated with ESAT-6 DNA, and their population further increased significantly by day 5. Moreover, the amount of tumor necrosis factor, which is an apoptosis-inducing factor produced mainly by DCs/macrophages, was greater in the ESAT-6 DNA treated tumors than in controls, and increased with repeat of the treatment. These results indicate that in vivo transfection of ESAT-6 DNA into tumor cells elicits significant inhibition of tumor growth by inducing potent activity of innate immunity mediated by DCs/macrophages, which may be followed by adaptive immunity against tumor associated antigens, elicited by the costimulation with ESAT-6 antigen. |
| تدمد: | 1347-7439 0916-7250 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f64c2395a6267b781815e184decce80d https://doi.org/10.1292/jvms.17-0466 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f64c2395a6267b781815e184decce80d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13477439 09167250 |
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