Methylated DNA and high total DNA levels in the serum of patients with breast cancer following neoadjuvant chemotherapy are predictive of a poor prognosis
| العنوان: | Methylated DNA and high total DNA levels in the serum of patients with breast cancer following neoadjuvant chemotherapy are predictive of a poor prognosis |
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| المؤلفون: | Shinzaburo Noguchi, Atsushi Shimomura, Naomi Maruyama, Yasuto Naoi, Seung Jin Kim, Koji Morimoto, Kenzo Shimazu, Noriaki Yamamoto, Masafumi Shimoda, Naofumi Oda, Naofumi Kagara, Noriko Fujita |
| المصدر: | Oncology Letters |
| بيانات النشر: | Spandidos Publications, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Cancer Research, medicine.medical_specialty, Pathology, medicine.medical_treatment, Gastroenterology, law.invention, chemistry.chemical_compound, breast cancer, Breast cancer, law, Internal medicine, medicine, Polymerase chain reaction, Chemotherapy, Oncogene, business.industry, Cancer, Articles, DNA, Cell cycle, medicine.disease, Molecular medicine, Oncology, chemistry, methylation, prognosis, business, serum, neoadjuvant chemotherapy |
| الوصف: | In a previous study, we established a one-step methylation-specific polymerase chain reaction (OS-MSP) assay for the detection of methylated DNA (met-DNA) and total DNA levels in serum. For the present study, this OS-MSP assay was used for patients with breast cancer treated with neoadjuvant chemotherapy (NAC) in order to investigate the prognostic significance of met-DNA and total DNA levels. Following treatment with NAC and prior to surgery, serum samples obtained from 120 patients with stage II/III breast cancer were subjected to the OS-MSP assay for analysis of the glutathione S-transferase pi 1, Ras association (RalGDS/AF-6) domain family member 1 and retinoic acid receptor β2 genes. The detection of methylation in a minimum of one of these genes indicated a positive outcome of the assay. The total DNA content of the serum was also determined. Of the 120 stage II/III patients, seven (6%) were positive for met-DNA in serum and showed a significantly worse overall survival (OS) time compared with patients negative for met-DNA (n=113) (5-year OS, 43 vs. 85%; P=0.002). The patients with high total DNA levels in serum (n=40) also showed a significantly worse OS compared with those with low total DNA levels (n=80) (65 vs. 91%; P |
| تدمد: | 1792-1082 1792-1074 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f766da9bea8f29313429c5ffe6552834 https://doi.org/10.3892/ol.2014.2068 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f766da9bea8f29313429c5ffe6552834 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17921082 17921074 |
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