Development of a reporter gene assay for antibody dependent cellular cytotoxicity activity determination of anti‐rabies virus glycoprotein antibodies
| العنوان: | Development of a reporter gene assay for antibody dependent cellular cytotoxicity activity determination of anti‐rabies virus glycoprotein antibodies |
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| المؤلفون: | Wenbo Wang, Chuanfei Yu, Yongfei Cui, Chunyu Liu, Yalan Yang, Gangling Xu, Gang Wu, Jialiang Du, Zhihao Fu, Luyong Guo, Caifeng Long, Xijie Xia, Yuhua Li, Lan Wang, Youchun Wang |
| المصدر: | Microbiology and Immunology. 67:69-78 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Virology, Immunology, Microbiology |
| الوصف: | Rabies is a viral disease that is nearly 100% fatal once clinical signs and symptoms develop. Post-exposure prophylaxis can efficiently prevent rabies, and antibody (Ab) induction by vaccination or passive immunization of human rabies immunoglobulin (HRIG) or monoclonal antibodies (mAbs) play an integral role in prevention against rabies. In addition to their capacity to neutralize viruses, antibodies exert their antiviral effects by antibody-dependent cellular cytotoxicity (ADCC), which plays an important role in antiviral immunity and clearance of viral infections. For antibodies against rabies virus (RABV), evaluation of ADCC activity was neglected. Here, we developed a robust cell-based reporter gene assay (RGA) for the determination of the ADCC activity of anti-RABV antibodies using CVS-N2c-293 cells, which stably express the glycoprotein (G) of RABV strain CVS-N2c as target cells, and Jurkat cells, which stably express FcγRⅢa and nuclear factor of activated T cells (NFAT) reporter gene as effector cells (Jurkat/NFAT-luc/FcγRⅢa cells). The experimental parameters were carefully optimized, and the established ADCC assay was systematically validated according to the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) Q2 guideline. We also evaluated the ADCC activity of anti-RABV antibodies, including mAbs, HRIG, and vaccine induced antisera, and found that all test antibodies exhibited ADCC activity with varied strengths. The established RGA provides a novel method for evaluating the ADCC of anti-RABV antibodies. |
| تدمد: | 1348-0421 0385-5600 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7b31a5023742b6b1cb97e1b73c531ea https://doi.org/10.1111/1348-0421.13036 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....f7b31a5023742b6b1cb97e1b73c531ea |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13480421 03855600 |
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