UPLC-QTOF/MS-Based Metabolomics Reveals the Protective Mechanism of Hydrogen on Mice with Ischemic Stroke
| العنوان: | UPLC-QTOF/MS-Based Metabolomics Reveals the Protective Mechanism of Hydrogen on Mice with Ischemic Stroke |
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| المؤلفون: | Pengchao Cheng, Yajuan Yang, Yufan Chao, Lilin Chen, Hongnan Zheng, Na Li |
| المصدر: | Neurochemical Research. 44:1950-1963 |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Taurine, Antioxidant, medicine.medical_treatment, Pharmacology, medicine.disease_cause, Biochemistry, Mass Spectrometry, Mice, 03 medical and health sciences, Cellular and Molecular Neuroscience, chemistry.chemical_compound, 0302 clinical medicine, medicine, Animals, Metabolomics, Chromatography, High Pressure Liquid, Phospholipids, chemistry.chemical_classification, Reactive oxygen species, Brain, Lysophosphatidylethanolamine, Infarction, Middle Cerebral Artery, General Medicine, Metabolism, Glutathione, Mitochondria, Oxidative Stress, Metabolic pathway, Neuroprotective Agents, 030104 developmental biology, chemistry, Reperfusion Injury, Energy Metabolism, 030217 neurology & neurosurgery, Oxidative stress, Hydrogen |
| الوصف: | As a reductive gas, hydrogen plays an antioxidant role by selectively scavenging oxygen free radicals. It has been reported that hydrogen has protective effects against nerve damage caused by ischemia-reperfusion in stroke, but the specific mechanism is still unclear. Therefore, this study aims to investigate the protective effects of hydrogen on stroke-induced ischemia-reperfusion injury and its detailed mechanism. Two weeks after the inhalation of high concentrations (66.7%) of hydrogen, middle cerebral artery occlusion (MCAO) was induced in mice using the thread occlusion technique to establish an animal model of the focal cerebral ischemia-reperfusion. Then, a metabolomics analysis of mouse cerebral cortex tissues was first performed by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) to study the metabolic changes and protective mechanisms of hydrogen on stroke ischemia-reperfusion injury. According to the metabolomic profiling of cortex tissues, 29 different endogenous metabolites were screened, including palmitoyl-L-carnitine, citric acid, glutathione, taurine, acetyl-L-carnitine, N-acetylaspartylglutamic acid (NAAG), L-aspartic acid, lysophosphatidylcholine (LysoPC) and lysophosphatidylethanolamine (LysoPE). Through pathway analysis, the metabolic pathways were concentrate on the glutathione pathway and the taurine pathway, mitochondrial energy metabolism and phospholipid metabolism that related to the oxidative stress process. This result reveals that hydrogen may protect against ischemic stroke by reducing oxidative stress during ischemia-reperfusion, thereby protecting nerve cells from reactive oxygen species(ROS). |
| تدمد: | 1573-6903 0364-3190 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f868a6c86f777a1758390bd7327b508f https://doi.org/10.1007/s11064-019-02829-x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....f868a6c86f777a1758390bd7327b508f |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15736903 03643190 |
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