Mutated human kirstenras, driven by a thyroglobulin promoter, induces a growth advantage and partially dedifferentiates rat thyroid epithelial cellsin vitro
| العنوان: | Mutated human kirstenras, driven by a thyroglobulin promoter, induces a growth advantage and partially dedifferentiates rat thyroid epithelial cellsin vitro |
|---|---|
| المؤلفون: | Daniela Califano, A. Mineo, V de Franciscis, Giancarlo Vecchio, Carmen Monaco, Giovanni Chiappetta, Giovanni Santelli |
| المساهمون: | Monaco, C, Califano, D, Chiappetta, G, Mineo, A, Defranciscis, V, Vecchio, Giancarlo, Santelli, G. |
| المصدر: | International Journal of Cancer. 63:757-760 |
| بيانات النشر: | Wiley, 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | EXPRESSION, endocrine system, Cancer Research, medicine.medical_specialty, endocrine system diseases, medicine.medical_treatment, Cellular differentiation, Molecular Sequence Data, Thyroid Gland, MURINE SARCOMA-VIRUS, ONCOGENE, Biology, Transfection, Thyroglobulin, Thyrotropin receptor, Internal medicine, medicine, Animals, Humans, Neoplastic transformation, Amino Acid Sequence, Promoter Regions, Genetic, MUTATIONS, Cell growth, Thyroid, Cell Differentiation, Epithelial Cells, GENE, TRANSFORMATION, Rats, Inbred F344, Rats, Cell Transformation, Neoplastic, Genes, ras, Endocrinology, medicine.anatomical_structure, Oncology, Mutation, Cancer research, Cell Division, Plasmids, Endocrine gland |
| الوصف: | We have earlier shown that expression of the human activated Ki-ras, directed by the rat thyroglobulin CTG) promoter in the thyroid gland of transgenic mice, is able to induce thyroid benign tumors, albeit at low incidence. A likely explanation of our results is that the low levels of exogenous Ki-ras transcripts are not sufficient to induce multifocal tumors in the thyroid gland. We have performed experiments to analyze the effects of a similar construct in vitro upon thyroid-cell proliferation and differentiation. Transfection of FRTL-5 rat thyroid cells with the human Ki-ras(val12) fused to the rat TG promoter is rapidly followed by reduced expression of the differentiation markers thyroglobulin, thyroperoxydase and thyrotropin receptor, but not by fully malignant cell transformation. The data reported support the hypothesis that Ki-ras mRNA levels are critical to the process of complete neoplastic transformation of thyroid epithelial differentiated cells in vitro. (C) 1995 Wiley-Liss, Inc. |
| وصف الملف: | STAMPA |
| تدمد: | 1097-0215 0020-7136 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f86ce6a310f29089d531319d2a16a203 https://doi.org/10.1002/ijc.2910630525 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....f86ce6a310f29089d531319d2a16a203 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970215 00207136 |
|---|