MicroRNA-200 family governs ovarian inclusion cyst formation and mode of ovarian cancer spread
| العنوان: | MicroRNA-200 family governs ovarian inclusion cyst formation and mode of ovarian cancer spread |
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| المؤلفون: | Pui-Wah Choi, Dale B. Hales, Kin Ming Kwan, William R. Welch, Shu-Kay Ng, Ross S. Berkowitz, Jamie S. L. Kwok, Karen Hales, Junzheng Yang, Ka Kui Tong, Shubai Liu, Shu-Wing Ng, Wing-Ping Fong, Christopher P. Crum, Stephen Kwok-Wing Tsui, Wai Wing So |
| المصدر: | Oncogene. 39:4045-4060 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, endocrine system, Cancer Research, Carcinogenesis, medicine.drug_class, media_common.quotation_subject, medicine.medical_treatment, Biology, 03 medical and health sciences, 0302 clinical medicine, microRNA, Genetics, medicine, Humans, RNA, Neoplasm, Molecular Biology, Ovulation, media_common, Ovarian Neoplasms, Regulation of gene expression, Gene knockdown, medicine.disease, Gene Expression Regulation, Neoplastic, Gene expression profiling, MicroRNAs, Ovarian Cysts, Steroid hormone, 030104 developmental biology, Estrogen, 030220 oncology & carcinogenesis, Cancer research, Female, Ovarian cancer |
| الوصف: | Epidemiologic and histopathologic findings and the laying hen model support the long-standing incessant ovulation hypothesis and cortical inclusion cyst involvement in sporadic ovarian cancer development. MicroRNA-200 (miR-200) family is highly expressed in ovarian cancer. Herewith, we show that ovarian surface epithelial (OSE) cells with ectopic miR-200 expression formed stabilized cysts in three-dimensional (3D) organotypic culture with E-cadherin fragment expression and steroid hormone pathway activation, whereas ovarian cancer 3D cultures with miR-200 knockdown showed elevated TGF-β expression, mitotic spindle disorientation, increased lumenization, disruption of ROCK-mediated myosin II phosphorylation, and SRC signaling, which led to histotype-dependent loss of collective movement in tumor spread. Gene expression profiling revealed that epithelial-mesenchymal transition and hypoxia were the top enriched gene sets regulated by miR-200 in both OSE and ovarian cancer cells. The molecular changes uncovered by the in vitro studies were verified in both human and laying hen ovarian cysts and tumor specimens. As miR-200 is also essential for ovulation, our results of estrogen pathway activation in miR-200-expressing OSE cells add another intriguing link between incessant ovulation and ovarian carcinogenesis. |
| تدمد: | 1476-5594 0950-9232 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f87354d3b1606a7527b3cdfd4e42fc8f https://doi.org/10.1038/s41388-020-1264-x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....f87354d3b1606a7527b3cdfd4e42fc8f |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14765594 09509232 |
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