Establishment and Validation of an In Vitro Screening Method for Traditional Chinese Medicine-Induced Nephrotoxicity
| العنوان: | Establishment and Validation of an In Vitro Screening Method for Traditional Chinese Medicine-Induced Nephrotoxicity |
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| المؤلفون: | Guo Xiao, Xuexiao Cao, Meng Wang, Rui Shao, Xiaoliang Ren, Yan Zhu, Zhe Ma, Ranran Dong |
| المصدر: | Evidence-Based Complementary and Alternative Medicine, Vol 2018 (2018) Evidence-based Complementary and Alternative Medicine : eCAM |
| بيانات النشر: | Hindawi Limited, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Kidney, Article Subject, business.industry, HEK 293 cells, lcsh:Other systems of medicine, Traditional Chinese medicine, Pharmacology, lcsh:RZ201-999, In vitro, Nephrotoxicity, 03 medical and health sciences, 030104 developmental biology, medicine.anatomical_structure, Complementary and alternative medicine, High-content screening, Medicine, Viability assay, business, Cytotoxicity, Research Article |
| الوصف: | Renal injury is among the adverse drug reactions (ADRs) caused by herbal medicine products (HMPs). Traditional Chinese medicines (TCMs) have been practiced for over 2000 years in China and East Asia, and herbs are currently used worldwide for the treatment and prevention of chronic and acute disease. Operetta high content analysis (HCA, PerkinElmer, Waltham, MA, USA), which is an in vitro, sensitive, reproducible, multiparametric screening method, was used to evaluate the cytotoxicity of HMPs in cultures of HEK293 human embryo kidney cells. Cytotoxic results were validated by an animal-based subacute toxicity assay. The renal safety of 18 active pharmaceutical agents from 13 TCM herbs with known nephrotoxic potential was evaluated in HEK293 human embryonic kidney cells. A panel of five parameters, cell viability, nuclear area, nuclear roundness, mitochondrial mass, and mitochondrial membrane potential, was utilized to evaluate drug-induced renal mitochondrial and nuclear injury. HCA can be a useful tool for preclinical screening and postclinical evaluation of HMPs. The nephrotoxicity of diosbulbin B and other HMPs was evident at a concentration as low as 0.01 μM. |
| وصف الملف: | text/xhtml |
| تدمد: | 1741-4288 1741-427X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8affdc63b811c4e7642b60527b206a0 https://doi.org/10.1155/2018/2461915 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f8affdc63b811c4e7642b60527b206a0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17414288 1741427X |
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