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An analytical strategy for quaternary ammonium neuromuscular blocking agents in a forensic setting using LC-MS/MS on a tandem quadrupole/time-of-flight instrument

التفاصيل البيبلوغرافية
العنوان: An analytical strategy for quaternary ammonium neuromuscular blocking agents in a forensic setting using LC-MS/MS on a tandem quadrupole/time-of-flight instrument
المؤلفون: Kevin D. Ballard, Fredric Rieders, Loan T. Nguyen, Francis X. Diamond, William E. Vickery
المصدر: Journal of the American Society for Mass Spectrometry. 17(10)
سنة النشر: 2006
مصطلحات موضوعية: Analyte, Analytical chemistry, Poison control, chemistry.chemical_compound, Biological specimen, Forensic Toxicology, Structural Biology, Tandem Mass Spectrometry, Humans, Sample preparation, Solid phase extraction, Enzyme Inhibitors, Succinylmonocholine, Spectroscopy, Chromatography, High Pressure Liquid, Brain Chemistry, Chromatography, Esterases, Heptafluorobutyric acid, Reference Standards, Neuromuscular Blocking Agents, Quaternary Ammonium Compounds, chemistry, Liver, Neuromuscular Agents, Indicators and Reagents
الوصف: An analytical strategy is described for analyzing quaternary ammonium neuromuscular blocking agents in a wide variety of biological specimens in a forensic setting. Neuromuscular blocking agents such as succinylcholine, pancuronium, and tubocurarine, often used as paralytic agents during surgery, are occasionally suspected as paralytic poisoning agents involved in suspected homicide and suicide cases. Because suspicion in such cases can develop slowly, the age, nature, and quality of available specimens varies greatly. The compounds are challenging analytically because of their simultaneous precharged yet lipophilic character. An analytical strategy has been devised for extracting these compounds from complex matrices using a combination of a modified Bligh and Dyer liquid-liquid extraction (used in reverse) followed by reverse-phase ion pairing solid-phase extraction using heptafluorobutyric acid as an ion pairing reagent. Final analysis is by LC-MS/MS using a tandem quadrupole orthogonal acceleration time of flight instrument (Q-TOF) with repetitive product ion scanning at high resolution. Native and spiked specimens are compared for both quantitative and especially qualitative purposes. The method has been applied to a wide variety of fluid and tissue specimen types, including numerous specimens from exhumation autopsies. For most specimens, detection limits are in the 2 to 10 ng/g range. Succinylmonocholine has been demonstrated to be present at low levels in normal posthumous kidney and liver. The Q-TOF is an excellent platform for forensic analytical investigations. This analytical strategy should also be applicable to other problematic analytes and sample matrices.
تدمد: 1044-0305
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8c101358144606c8d94ea5c4f074446
https://pubmed.ncbi.nlm.nih.gov/16887364
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....f8c101358144606c8d94ea5c4f074446
قاعدة البيانات: OpenAIRE
الوصف
تدمد:10440305