egfl6 expression in the pharyngeal pouch is dispensable for craniofacial development
| العنوان: | egfl6 expression in the pharyngeal pouch is dispensable for craniofacial development |
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| المؤلفون: | Jangwon Park, Hyejee Na, Haewon Jeon, Sil Jin, Chong Pyo Choe |
| المصدر: | Animal Cells and Systems article-version (VoR) Version of Record Animal Cells and Systems, Vol 25, Iss 5, Pp 255-263 (2021) |
| بيانات النشر: | Taylor & Francis, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Basement membrane, Medicine (General), integumentary system, Pharyngeal pouch, biology, QH301-705.5, Angiogenesis, egfl6, Articles, pharyngeal pouch, craniofacial development, zebrafish, biology.organism_classification, General Biochemistry, Genetics and Molecular Biology, Cell biology, R5-920, medicine.anatomical_structure, Hair follicle morphogenesis, Notochord, medicine, Animal Science and Zoology, Biology (General), Craniofacial, Zebrafish, Research Article |
| الوصف: | Epidermal growth factor-like domain multiple 6 (Egfl6) is a basement membrane protein and plays an important role in hair follicle morphogenesis, angiogenesis, notochord development in vertebrates. Although egfl6 expression in the developing head was observed in zebrafish, its role for craniofacial development and the determination of the pharyngeal region expressing egfl6, have not been reported yet. Here, we report the expression patterns and function of egfl6 in craniofacial development in zebrafish. egfl6 was expressed sequentially in the developing pharyngeal pouches that are key epithelial structures governing the development of the vertebrate head. However, loss-of-function mutations in egfl6 did not cause any craniofacial defects, including the pouches as well as the thymus and facial cartilages whose development is contingent upon appropriate pouch formation. egfl6 was unlikely redundant with egfl7 expressed in a distinct pharyngeal region from that of egfl6 in craniofacial development because reduction of egfl7 with a MO in egfl6 mutants did not affect craniofacial development. In addition, we found that egfl6 carried an endogenous start loss mutation in the wild-type T��bingen strain, implying egfl6 would be a non-functional gene. Taken all together, we suggest that egfl6 expression in the pharyngeal pouches is not required for craniofacial development in zebrafish. |
| DOI: | 10.6084/m9.figshare.16522585.v1 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8fc8627b12a8b9e4e290dd3157f33e3 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f8fc8627b12a8b9e4e290dd3157f33e3 |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.6084/m9.figshare.16522585.v1 |
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