Direct analysis of hydroxylated polycyclic aromatic hydrocarbons in biological samples with complex matrices using polarity‐reversed nanoelectrospray ionization
| العنوان: | Direct analysis of hydroxylated polycyclic aromatic hydrocarbons in biological samples with complex matrices using polarity‐reversed nanoelectrospray ionization |
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| المؤلفون: | Siyuan Tan, Juduo Wang, Manli Li, Xinchi Yin, Xiaomei Gao, Chang Li, You Jiang, Xinhua Dai, Xiaoyun Gong, Xiang Fang |
| المصدر: | Rapid Communications in Mass Spectrometry. 36 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Serum, Spectrometry, Mass, Electrospray Ionization, Molecular Structure, Organic Chemistry, Humans, Environmental Pollutants, Polycyclic Aromatic Hydrocarbons, Urine, Hydroxylation, Sensitivity and Specificity, Spectroscopy, Analytical Chemistry |
| الوصف: | Polycyclic aromatic hydrocarbons (PAHs) are a class of environmental contaminants with carcinogenic effect drawing worldwide attention. PAHs can be converted into hydroxylated PAHs (OH-PAHs) through metabolic processes. Thus, they are commonly considered as an important class of biomarkers of PAH exposure. However, direct analysis of related metabolites of these environmental pollutants in biological samples using mass spectrometry is still challenging because of matrix effect and ion suppression during nanoelectrospray ionization (nano-ESI).In our previous work, a polarity-reversed nanoelectrospray ionization (PR-nESI) technique was developed for the analysis of biomolecules in complex matrices. In this work, we further optimized PR-nESI for direct and sensitive analysis of OH-PAHs in different samples under severe salt interference in negative polarity.Compared with conventional nano-ESI, the optimized PR-nESI method realized sensitive detection of 1-naphthol in samples with a concentration of salt up to millimolar level. The signal-to-noise ratio (S/N) of OH-PAHs was increased by 1-2 orders of magnitude compared with conventional nano-ESI. Six different OH-PAHs were successfully detected with high S/N ratio using PR-nESI. PR-nESI was further successfully applied in the analysis of OH-PAHs in spiked fetal blood serum, human urine, and single-cell samples. For environmentally exposed subjects, the detections of OH-PAHs in single-cell samples and urines from human smokers were successfully conducted.The optimized PR-nESI method was successfully applied for the sensitive analysis of OH-PAHs in complex biological samples with severe salt effects. Based on the present study, PR-nESI can have a promising prospect for the sensitive analysis of other metabolites of environmental pollutants in negative polarity. |
| تدمد: | 1097-0231 0951-4198 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f953a353b183501e39c7e563bc796ca7 https://doi.org/10.1002/rcm.9237 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....f953a353b183501e39c7e563bc796ca7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970231 09514198 |
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