Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells
| العنوان: | Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells |
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| المؤلفون: | Niklas Arnberg, Linda Sandblad, B. David Persson, Yoshikazu Takada, Jason Gall, Lynn M. Schnapp, Jyrki Heino, Lars Frängsmyr, A. Paul Mould, Annelie Olofsson, Anandi Rajan |
| المصدر: | Scientific reports, vol 8, iss 1 Scientific Reports, Vol 8, Iss 1, Pp 1-14 (2018) Scientific Reports |
| بيانات النشر: | eScholarship, University of California, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Adenoviruses, media_common.quotation_subject, viruses, Integrin, lcsh:Medicine, Virus Attachment, CHO Cells, Integrin alpha6, Article, Microbiology in the medical area, Cell Line, 03 medical and health sciences, Transduction (genetics), Cricetulus, Receptors, Mikrobiologi inom det medicinska området, Animals, Humans, Internalization, Receptor, Laminin binding, lcsh:Science, media_common, RGD motif, Integrin alpha6beta4, Multidisciplinary, biology, Chemistry, Adenoviruses, Human, lcsh:R, virus diseases, Surface Plasmon Resonance, biology.organism_classification, eye diseases, 3. Good health, Cell biology, Virus, 030104 developmental biology, Cell culture, biology.protein, Receptors, Virus, lcsh:Q, Laminin, HT29 Cells, Human, Biotechnology |
| الوصف: | The enteric species F human adenovirus types 40 and 41 (HAdV-40 and -41) are the third most common cause of infantile gastroenteritis in the world. Knowledge about HAdV-40 and -41 cellular infection is assumed to be fundamentally different from that of other HAdVs since HAdV-40 and -41 penton bases lack the αV-integrin-interacting RGD motif. This motif is used by other HAdVs mainly for internalization and endosomal escape. We hypothesised that the penton bases of HAdV-40 and -41 interact with integrins independently of the RGD motif. HAdV-41 transduction of a library of rodent cells expressing specific human integrin subunits pointed to the use of laminin-binding α2-, α3- and α6-containing integrins as well as other integrins as candidate co-receptors. Specific laminins prevented internalisation and infection, and recombinant, soluble HAdV-41 penton base proteins prevented infection of human intestinal HT-29 cells. Surface plasmon resonance analysis demonstrated that HAdV-40 and -41 penton base proteins bind to α6-containing integrins with an affinity similar to that of previously characterised penton base:integrin interactions. With these results, we propose that laminin-binding integrins are co-receptors for HAdV-40 and -41. Originally included in thesis in manuscript form. |
| وصف الملف: | application/pdf |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f97739e1ae78a5df5c2c8ff0de437706 https://escholarship.org/uc/item/2jx6j8p4 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f97739e1ae78a5df5c2c8ff0de437706 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |