Phytochemical characterization and anti-inflammatory activity of a water extract of Gentiana purpurea roots
| العنوان: | Phytochemical characterization and anti-inflammatory activity of a water extract of Gentiana purpurea roots |
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| المؤلفون: | Lin Zhang, Emilie Steinbakk Ulriksen, Håvard Hoel, Lene Sandvik, Karl Egil Malterud, Kari Tvete Inngjerdingen, Marit Inngjerdingen, Helle Wangensteen |
| المصدر: | Journal of ethnopharmacology. 301 |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Pharmacology, Tumor Necrosis Factor-alpha, Plant Extracts, Drug Discovery, Phytochemicals, Leukocytes, Mononuclear, Anti-Inflammatory Agents, Water, Gentiana, Plant Roots |
| الوصف: | Ethnopharmacological relevance Gentiana purpurea was one of the most important medicinal plants in Norway during the 18th and 19th centuries, and the roots were used against different types of gastrointestinal and airway diseases. Aim of the study To explore the content of bioactive compounds in a water extract from the roots, a preparation commonly used in traditional medicine in Norway, to assess the anti-inflammatory potential, and furthermore to quantify the major bitter compounds in both roots and leaves. Materials and methods G. purpurea roots were boiled in water, the water extract applied on a Diaion HP20 column and further fractionated with Sephadex LH20, reverse phase C18 and normal phase silica gel to obtain the low molecular compounds. 1D NMR, 2D NMR, and ESI-MS were used for structure elucidation. HPLC-DAD analysis was used for quantification. The inhibition of TNF-α secretion in ConA stimulated peripheral blood mononuclear cells (PBMCs) was investigated. Results Eleven compounds were isolated and identified from the hot water extract of G. purpurea roots. Gentiopicrin, amarogentin, erythrocentaurin and gentiogenal showed dose-dependent inhibition of TNF-α secretion. Gentiopicrin is the major secondary metabolite in the roots, while sweroside dominates in the leaves. Conclusions The present work gives a comprehensive overview of the major low-molecular weight compounds in the water extracts of G. purpurea, including metabolites produced during the decoction process, and show new anti-inflammatory activities for the native bitter compounds as well as the metabolites produced during preparation of the crude drug. |
| تدمد: | 1872-7573 0378-8741 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f985aa4f1a322bdb6c8150a0c1ea9b7c https://pubmed.ncbi.nlm.nih.gov/36220509 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f985aa4f1a322bdb6c8150a0c1ea9b7c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18727573 03788741 |
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