Surface Labeling with Adhesion Protein FimH Improves Binding of Immunotherapeutic Agent Salmonella Ty21a to the Bladder Epithelium
| العنوان: | Surface Labeling with Adhesion Protein FimH Improves Binding of Immunotherapeutic Agent Salmonella Ty21a to the Bladder Epithelium |
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| المؤلفون: | Maroeska J. Burggraaf, Diane Houben, Coen Kuijl, Mariska Verlaan, J.J. Maaskant, Joen Luirink, Lisette Waanders, Carla F. M. Molthoff, Wilbert Bitter |
| المساهمون: | Medical Microbiology and Infection Prevention, AII - Infectious diseases, Molecular Microbiology, AIMMS, LaserLaB - Molecular Biophysics |
| المصدر: | Bladder Cancer, 7(1), 79-90. IOS Press Burggraaf, M J, Waanders, L, Verlaan, M, Maaskant, J, Houben, D, Luirink, J, Bitter, W, Kuijl, C & Molthoff, C F M 2021, ' Surface Labeling with Adhesion Protein FimH Improves Binding of Immunotherapeutic Agent Salmonella Ty21a to the Bladder Epithelium ', Bladder Cancer, vol. 7, no. 1, pp. 79-90 . https://doi.org/10.3233/blc-200382, https://doi.org/10.3233/BLC-200382 Burggraaf, M J, Waanders, L, Verlaan, M, Maaskant, J, Houben, D, Luirink, J, Bitter, W, Kuijl, C & Molthoff, C F M 2021, ' Surface Labeling with Adhesion Protein FimH Improves Binding of Immunotherapeutic Agent Salmonella Ty21a to the Bladder Epithelium ', Bladder Cancer, vol. 7, no. 1, pp. 79-90 . https://doi.org/10.3233/BLC-200382 |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Urology, medicine.medical_treatment, Ty21a, complex mixtures, 03 medical and health sciences, 0302 clinical medicine, SDG 3 - Good Health and Well-being, In vivo, medicine, Bladder cancer, biology, business.industry, Ty21a typhoid vaccine, E. coli, Cancer, Salmonella enterica, Immunotherapy, biology.organism_classification, medicine.disease, In vitro, Epithelium, 030104 developmental biology, medicine.anatomical_structure, Oncology, 030220 oncology & carcinogenesis, Cancer research, bladder cancer, immunotherapy, FimH protein, business |
| الوصف: | BACKGROUND: Bladder cancer is the ninth most common cancer in men. 70% of these tumors are classified as non-muscle invasive bladder cancer and those patients receive 6 intravesical instillations with Mycobacterium bovis BCG after transurethral resection. However, 30% of patients show recurrences after treatment and experience severe side effects that often lead to therapy discontinuation. Recently, another vaccine strain, Salmonella enterica typhi Ty21a, demonstrated promising antitumor activity in vivo. Here we focus on increasing bacterial retention in the bladder in order to reduce the number of instillations required and improve antitumor activity. OBJECTIVE: To increase the binding of Ty21a to the bladder wall by surface labeling of the bacteria with adhesion protein FimH and to study its effect in a bladder cancer mouse model. METHODS: Binding of Ty21a with surface-labeled FimH to the bladder wall was analyzed in vitro and in vivo. The antitumor effect of a single instillation of Ty21a+FimH in treatment was determined in a survival experiment. RESULTS: FimH-labeled Ty21a showed significant (p |
| اللغة: | English |
| تدمد: | 2352-3727 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f99173ae400ed56af37971eec3203de9 https://doi.org/10.3233/blc-200382 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f99173ae400ed56af37971eec3203de9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23523727 |
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