Investigation of olfactory receptor family 51 subfamily j member 1 (OR51J1) gene susceptibility as a potential breast cancer-associated biomarker
| العنوان: | Investigation of olfactory receptor family 51 subfamily j member 1 (OR51J1) gene susceptibility as a potential breast cancer-associated biomarker |
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| المؤلفون: | Ali Akbar Jafari, Nahid Ahmadi, Maryam Asadi, Nasrollah Erfani, Simin Ahmadvand, Akbar Safaei, Amin Ramezani |
| المصدر: | PLoS ONE, Vol 16, Iss 2, p e0246752 (2021) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | medicine.medical_treatment, Protein Expression, Cancer Treatment, Gene Expression, Receptors, Odorant, Biochemistry, Targeted therapy, Breast Tumors, Databases, Genetic, Medicine and Health Sciences, Medicine, Multidisciplinary, Invasive Tumors, Prostate Cancer, Liver Diseases, Prostate Diseases, Immunohistochemistry, Up-Regulation, Gene Expression Regulation, Neoplastic, medicine.anatomical_structure, Oncology, Biomarker (medicine), Female, Research Article, Urology, Science, Human Protein Atlas, Breast Neoplasms, Gastroenterology and Hepatology, Research and Analysis Methods, Real-Time Polymerase Chain Reaction, Breast cancer, Breast Cancer, Gastrointestinal Tumors, Gene Expression and Vector Techniques, Genetics, Biomarkers, Tumor, Humans, Genetic Predisposition to Disease, Molecular Biology Techniques, Molecular Biology, Molecular Biology Assays and Analysis Techniques, Olfactory receptor, business.industry, Carcinoma, Myoepithelial cell, Cancer, Cancers and Neoplasms, Biology and Life Sciences, Hepatocellular Carcinoma, medicine.disease, Genitourinary Tract Tumors, Cancer research, business, Biomarkers |
| الوصف: | Among cancer treatment methods, targeted therapy using cancer-associated biomarkers has minimum side effects. Recently olfactory receptor (OR) family attracts the researcher’s attention as a favorable biomarker of cancer. Here, a statistical approach using complete data from the human protein atlas database was used to evaluate the potential of OR51J1 gene as a cancer-associated biomarker. To confirm the findings of statistical analysis, the OR51J1 mRNA and protein expression levels in breast tumor and normal tissue were measured using quantitative Real Time PCR (qRT-PCR) and immunohistochemistry (IHC) techniques. The association with clinicopathological factors was analyzed. Statistical analysis revealed that OR51J1 has a high expression level in more than 20 types of cancer tissues without any expression in 44 normal tissues. In 15 cancer types, including breast cancer, expression score was more than 90%. The qRT-PCR analysis in breast cancer showed OR51J1 have significantly higher expression level in tumors than normal tissues (2.91 fold). The IHC results showed OR51J1 expression on other cellular subtypes than tumor and normal cells, including myoepithelium, fibroblast, and lymphocytes. OR51J1 protein expression in invasive cells, as well as its overall score, showed a significant correlation with ER and PR expression and breast cancer (BC) subtypes. Results revealed the potential of OR51J1 as a cancer-associated biomarker for the diagnosis of breast cancer at the mRNA level. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f9a3a5a25e9579673f31571b66021e6d https://doaj.org/article/881cfcb56ca54c689cbe2dfda38c786a |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f9a3a5a25e9579673f31571b66021e6d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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