3D-localization microscopy and tracking of FoF1-ATP synthases in living bacteria
| العنوان: | 3D-localization microscopy and tracking of FoF1-ATP synthases in living bacteria |
|---|---|
| المؤلفون: | Anja Renz, Marc Renz, Michael Börsch, Diana Klütsch, Gabriele Deckers-Hebestreit |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | ATP synthase, biology, Chemistry, Biomolecules (q-bio.BM), Tracking (particle physics), medicine.disease_cause, biology.organism_classification, chemistry.chemical_compound, Membrane, Quantitative Biology - Biomolecules, Membrane curvature, FOS: Biological sciences, Microscopy, biology.protein, medicine, Biophysics, Escherichia coli, Adenosine triphosphate, Bacteria |
| الوصف: | FoF1-ATP synthases are membrane-embedded protein machines that catalyze the synthesis of adenosine triphosphate. Using photoactivation-based localization microscopy (PALM) in TIR-illumination as well as structured illumination microscopy (SIM), we explore the spatial distribution and track single FoF1-ATP synthases in living E. coli cells under physiological conditions at different temperatures. For quantitative diffusion analysis by mean-squared-displacement measurements, the limited size of the observation area in the membrane with its significant membrane curvature has to be considered. Therefore, we applied a 'sliding observation window' approach (M. Renz et al., Proc. SPIE 8225, 2012) and obtained the one-dimensional diffusion coefficient of FoF1-ATP synthase diffusing on the long axis in living E. coli cells. 13 pages, 5 figures |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f9d1a4bba89b2b083f5b28aed79022e1 http://arxiv.org/abs/1502.00544 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f9d1a4bba89b2b083f5b28aed79022e1 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |