Association of miR-146a, miR-149, miR-196a2, and miR-499 Polymorphisms with Ossification of the Posterior Longitudinal Ligament of the Cervical Spine
| العنوان: | Association of miR-146a, miR-149, miR-196a2, and miR-499 Polymorphisms with Ossification of the Posterior Longitudinal Ligament of the Cervical Spine |
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| المؤلفون: | Hyoung Sik Min, Jang Bo Lee, Jeon Young-Joo, Keung Nyun Kim, Seil Sohn, Jung Oh Kim, Jae Joon Lim, Ok Joon Kim, Hemant Kumar, Alexander E. Ropper, Sung Uk Kuh, Nam Keun Kim, Dong Ah Shin, Inbo Han |
| المصدر: | PLoS ONE PLOS ONE(11): 7 PLoS ONE, Vol 11, Iss 7, p e0159756 (2016) |
| بيانات النشر: | Public Library of Science (PLoS), 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Oncology, Heredity, Bone density, Physiology, lcsh:Medicine, Blood Pressure, Polygenic disease, Ossification, Ossification of Posterior Longitudinal Ligament, Pathology and Laboratory Medicine, Biochemistry, Vascular Medicine, Endocrinology, Gene Frequency, Bone Density, Medicine and Health Sciences, Medicine, lcsh:Science, Immune Response, Multidisciplinary, Ossification of the posterior longitudinal ligament, Anatomy, Middle Aged, Nucleic acids, Genetic Mapping, Connective Tissue, Hypertension, Cervical Vertebrae, Female, Bone Remodeling, medicine.symptom, Research Article, Adult, medicine.medical_specialty, Genotype, Endocrine Disorders, Immunology, Variant Genotypes, Single-nucleotide polymorphism, Polymorphism, Single Nucleotide, 03 medical and health sciences, Signs and Symptoms, Asian People, Diagnostic Medicine, Internal medicine, Republic of Korea, microRNA, Genetics, Diabetes Mellitus, Humans, Genetic Predisposition to Disease, Non-coding RNA, Bone, Alleles, Aged, Inflammation, Ligaments, Biology and life sciences, business.industry, lcsh:R, Cervical spine, Gene regulation, MicroRNAs, Biological Tissue, 030104 developmental biology, Haplotypes, Metabolic Disorders, Case-Control Studies, RNA, lcsh:Q, Gene expression, Physiological Processes, business, Mir 196a2 |
| الوصف: | Background Ossification of the posterior longitudinal ligament (OPLL) of the spine is considered a multifactorial and polygenic disease. We aimed to investigate the association between four single nucleotide polymorphisms (SNPs) of pre-miRNAs [miR-146aC>G (rs2910164), miR-149T>C (rs2292832), miR-196a2T>C (rs11614913), and miR-499A>G (rs3746444)] and the risk of cervical OPLL in the Korean population. Methods The genotypic frequencies of these four SNPs were analyzed in 207 OPLL patients and 200 controls by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Findings For four SNPs in pre-miRNAs, no significant differences were found between OPLL patients and controls. However, subgroup analysis based on OPLL subgroup (continuous: continuous type plus mixed type, segmental: segmental and localized type) showed that miR-499GG genotype was associated with an increased risk of segmental type OPLL (adjusted odds ratio = 4.314 with 95% confidence interval: 1.109–16.78). In addition, some allele combinations (C-T-T-G, G-T-T-A, and G-T-C-G of miR-146a/-149/-196a2/-499) and combined genotypes (miR-149TC/miR-196a2TT) were associated with increased OPLL risk, whereas the G-T-T-G and G-C-C-G allele combinations were associated with decreased OPLL risk. Conclusion The results indicate that GG genotype of miR-499 is associated with significantly higher risks of OPLL in the segmental OPLL group. The miR-146a/-149/-196a2/-499 allele combinations may be a genetic risk factor for cervical OPLL in the Korean population. |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f9ef68e5824146740188b5999d6dc947 https://doi.org/10.1371/journal.pone.0159756 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f9ef68e5824146740188b5999d6dc947 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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