Deletion status of p16 in effusion smear preparation correlates with that of underlying malignant pleural mesothelioma tissue
| العنوان: | Deletion status of p16 in effusion smear preparation correlates with that of underlying malignant pleural mesothelioma tissue |
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| المؤلفون: | Kunimitsu Kawahara, Kenzo Hiroshima, Makoto Hamasaki, Kenichi Taguchi, Tomoyuki Hida, Yoshinao Oda, Hiroshi Honda, Tohru Tsujimura, Toshiaki Kamei, Shinji Matsumoto, Kazuki Nabeshima, Akinori Iwasaki |
| المصدر: | Cancer Science |
| بيانات النشر: | John Wiley and Sons Inc., 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, Mesothelioma, Cancer Research, Pathology, medicine.medical_specialty, Lung Neoplasms, Pleural effusion, Pleural Neoplasms, p16, Malignancy, Diagnosis, Differential, Mesothelial hyperplasia, medicine, Biomarkers, Tumor, malignant pleural mesothelioma, Humans, Pleural Neoplasm, fluorescence in situ hybridization, In Situ Hybridization, Fluorescence, Aged, Aged, 80 and over, Hyperplasia, medicine.diagnostic_test, business.industry, reactive mesothelial hyperplasia, Genes, p16, Mesothelioma, Malignant, General Medicine, Original Articles, Middle Aged, Pleural Diseases, medicine.disease, Pleural Effusion, Oncology, Effusion, Cytogenetic Analysis, Cancer research, Original Article, Female, business, Cytology, Fluorescence in situ hybridization |
| الوصف: | Differentiating malignant pleural mesothelioma (MPM) cells morphologically from reactive mesothelial hyperplasia cells is problematic. Homozygous deletion (HD) of p16 (CDKN2A), detected by FISH, is a good marker of malignancy and is useful to differentiate between these cells. However, the correlation between the p16 status of effusion smears and that of the underlying MPM tissues has not been investigated. We used p16-specific FISH to investigate 20 cases of MPM from which both effusion cytologic smears and histologic specimens were available. In five cases, histologic specimens included both an invasive component and surface mesothelial proliferation. In 14 cases (70%), MPM cells in both tissue sections and effusion smears were p16 HD-positive. Conversely, MPM cells in the remaining six tumors (30%) were p16 HD-negative in both tissue sections and effusion smears. For all five MPM cases with surface mesothelial proliferations and invasive components, the effusion smears, surface mesothelial proliferations, and invasive MPM components all displayed p16 deletion. Moreover, the extent to which p16 was deleted in smears highly correlated with the extent of p16 deletion in tissues. The p16 deletion percentages were also similar among smears, tissue surface proliferations, and invasive components. In cases with clinical and radiologic evidence of a diffuse pleural tumor, detection of p16 deletion in cytologic smear samples may permit MPM diagnosis without additional tissue examination. However, the absence of p16 deletion in cytologic smear samples does not preclude MPM. |
| اللغة: | English |
| تدمد: | 1349-7006 1347-9032 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa7a58c56890981261604d8bb724c105 http://europepmc.org/articles/PMC4714681 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....fa7a58c56890981261604d8bb724c105 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13497006 13479032 |
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