Straightforward Generation of Ultrapure Off-the-Shelf Allogeneic CAR-T Cells
| العنوان: | Straightforward Generation of Ultrapure Off-the-Shelf Allogeneic CAR-T Cells |
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| المؤلفون: | Ming Yang, Julien Valton, Diane Tkach, Philippe Duchateau, Alexandre Juillerat, Alex Boyne, Laurent Poirot |
| المصدر: | Frontiers in Bioengineering and Biotechnology Frontiers in Bioengineering and Biotechnology, Vol 8 (2020) |
| بيانات النشر: | Frontiers Media S.A., 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Histology, lcsh:Biotechnology, Cell, Adoptive immunotherapy, Population, Biomedical Engineering, Bioengineering, 02 engineering and technology, cell purification, mRNA vectorization, 03 medical and health sciences, lcsh:TP248.13-248.65, medicine, Off the shelf, education, GvHD, education.field_of_study, Chemistry, T-cell receptor, Bioengineering and Biotechnology, Brief Research Report, 021001 nanoscience & nanotechnology, Cell biology, chimeric antigen receptor (CAR) cells, 030104 developmental biology, medicine.anatomical_structure, Car t cells, 0210 nano-technology, human activities, adoptive immunotherapy, Biotechnology |
| الوصف: | Here, we developed a straightforward methodology to generate TCRαβ negative (allogeneic) cells for CAR-T cell therapies. With an early and transient expression of an anti-CD3 CAR in the engineered donor T cells, we programmed these cells to self-eliminate the TCR+ cell population and obtained an ultrapure TCRαβ- population (99-99.9%) at the end of the CAR-T production. This novel and easy-to-implement procedure preserves the production yield and cell fitness and has the potential to streamline the manufacturing of "off-the-shelf" CAR T-cell therapies. |
| اللغة: | English |
| تدمد: | 2296-4185 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa84b3aae6f2453002ffa876af2192e1 http://europepmc.org/articles/PMC7330105 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....fa84b3aae6f2453002ffa876af2192e1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22964185 |
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