أعرض في EDS

Long noncoding RNA SBF2-AS1 act as a ceRNA to modulate cell proliferation via binding with miR-188-5p in acute myeloid leukemia

التفاصيل البيبلوغرافية
العنوان: Long noncoding RNA SBF2-AS1 act as a ceRNA to modulate cell proliferation via binding with miR-188-5p in acute myeloid leukemia
المؤلفون: Jia Guo, Peiling Li, Dongju Zhao, Ai-Ju Xiao, Yun-Jiao Tian, Tuanjie Wang, Yanhua Wang
المصدر: Artificial cells, nanomedicine, and biotechnology. 47(1)
سنة النشر: 2019
مصطلحات موضوعية: Ubiquitin-Protein Ligases, Biomedical Engineering, Pharmaceutical Science, Medicine (miscellaneous), 02 engineering and technology, Biology, Flow cytometry, 03 medical and health sciences, Mice, 0302 clinical medicine, Downregulation and upregulation, Cell Movement, hemic and lymphatic diseases, Cell Line, Tumor, medicine, Animals, Humans, neoplasms, Cell Proliferation, medicine.diagnostic_test, Base Sequence, Competing endogenous RNA, Cell growth, Myeloid leukemia, General Medicine, 021001 nanoscience & nanotechnology, In vitro, Long non-coding RNA, Up-Regulation, Leukemia, Myeloid, Acute, MicroRNAs, Cell Transformation, Neoplastic, Apoptosis, 030220 oncology & carcinogenesis, Cancer research, Female, RNA, Long Noncoding, 0210 nano-technology, Biotechnology
الوصف: LncRNA SBF2-AS1 has been reported to be implicated in the deterioration of multiple human cancers. However, the roles and underlying mechanisms of SBF2-AS1 in acute myeloid leukemia (AML) are still unclear. In the present study, the online GEPIA database showed that SBF2-AS1 expression was significantly increased in AML samples. QRT-PCR results showed that SBF2-AS1 expression was upregulated in AML cells. CCK-8 assay revealed that SBF2-AS1 inhibition decreased AML cells proliferation ability in vitro. Flow cytometry assays showed that SBF2-AS1 inhibition induced AML cells apoptosis and arrested AML cells in G0/G1 phase. Mechanistically, miR-188-5p was identified as a direct target of SBF2-AS1. SBF2-AS1 upregulated the expression level of ZFP91 by sponging miR-188-5p. And the effects of SBF2-AS1 suppression on AML cells progression could be abolished by miR-188-5p inhibitors. Moreover, we found that SBF2-AS1 inhibition reduced tumor growth in vivo. Taken together, our findings elucidated that SBF2-AS1 could act as a miRNA sponge in AML progression, and provided a potential therapeutic strategy for AML treatment.
تدمد: 2169-141X
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa8e5293bdade40b058852944e8d637c
https://pubmed.ncbi.nlm.nih.gov/31062614
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....fa8e5293bdade40b058852944e8d637c
قاعدة البيانات: OpenAIRE
الوصف
تدمد:2169141X