Impairment of spermatogenesis and sperm motility by the high-fat diet-induced dysbiosis of gut microbes
| العنوان: | Impairment of spermatogenesis and sperm motility by the high-fat diet-induced dysbiosis of gut microbes |
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| المؤلفون: | Ning Ding, Yun Jing Yan, Fang Hong Li, Bing Yao, Yun Ping Mu, Geng Miao Xiao, Allan Z. Zhao, Jun Jing, Xin Zhang, Li Li Peng, Xue Di Zhang, Hao Chen, Shan Shan Liu, Xin Yun Bi |
| المصدر: | Gut. 69:1608-1619 |
| بيانات النشر: | BMJ, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Normal diet, T-Lymphocytes, Prevotella, Motility, Gut flora, Diet, High-Fat, Andrology, Feces, Mice, 03 medical and health sciences, 0302 clinical medicine, medicine, Animals, Bacteroides, Humans, Correlation of Data, Spermatogenesis, Sperm motility, Epididymis, 030219 obstetrics & reproductive medicine, biology, Macrophages, Gastroenterology, food and beverages, biology.organism_classification, medicine.disease, Sperm, Endotoxemia, Gastrointestinal Microbiome, Transplantation, 030104 developmental biology, Sperm Motility, Cytokines, Dysbiosis |
| الوصف: | ObjectiveHigh-fat diet (HFD)-induced metabolic disorders can lead to impaired sperm production. We aim to investigate if HFD-induced gut microbiota dysbiosis can functionally influence spermatogenesis and sperm motility.DesignFaecal microbes derived from the HFD-fed or normal diet (ND)-fed male mice were transplanted to the mice maintained on ND. The gut microbes, sperm count and motility were analysed. Human faecal/semen/blood samples were collected to assess microbiota, sperm quality and endotoxin.ResultsTransplantation of the HFD gut microbes into the ND-maintained (HFD-FMT) mice resulted in a significant decrease in spermatogenesis and sperm motility, whereas similar transplantation with the microbes from the ND-fed mice failed to do so. Analysis of the microbiota showed a profound increase in genus Bacteroides and Prevotella, both of which likely contributed to the metabolic endotoxaemia in the HFD-FMT mice. Interestingly, the gut microbes from clinical subjects revealed a strong negative correlation between the abundance of Bacteroides-Prevotella and sperm motility, and a positive correlation between blood endotoxin and Bacteroides abundance. Transplantation with HFD microbes also led to intestinal infiltration of T cells and macrophages as well as a significant increase of pro-inflammatory cytokines in the epididymis, suggesting that epididymal inflammation have likely contributed to the impairment of sperm motility. RNA-sequencing revealed significant reduction in the expression of those genes involved in gamete meiosis and testicular mitochondrial functions in the HFD-FMT mice.ConclusionWe revealed an intimate linkage between HFD-induced microbiota dysbiosis and defect in spermatogenesis with elevated endotoxin, dysregulation of testicular gene expression and localised epididymal inflammation as the potential causes.Trial registration numberNCT03634644. |
| تدمد: | 1468-3288 0017-5749 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa93cf23c4c38fe1b22d5af3aa1ad9c5 https://doi.org/10.1136/gutjnl-2019-319127 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....fa93cf23c4c38fe1b22d5af3aa1ad9c5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14683288 00175749 |
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