A Novel Transcript for the Thyrotropin-Releasing Hormone Receptor in Human Pituitary and Pituitary Tumors
| العنوان: | A Novel Transcript for the Thyrotropin-Releasing Hormone Receptor in Human Pituitary and Pituitary Tumors |
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| المؤلفون: | Masatomo Mori, Nobuyuki Shibusawa, Syozo Yamada, Koushi Hashimoto, Teturou Satoh, Hideaki Kohga, Masanobu Yamada, Yasunori Ozawa |
| المصدر: | The Journal of Clinical Endocrinology & Metabolism. 82:4224-4228 |
| بيانات النشر: | The Endocrine Society, 1997. |
| سنة النشر: | 1997 |
| مصطلحات موضوعية: | Adenoma, Adult, Male, Pituitary gland, medicine.medical_specialty, DNA, Complementary, Transcription, Genetic, Endocrinology, Diabetes and Metabolism, Molecular Sequence Data, Clinical Biochemistry, CHO Cells, Peptide hormone, Biology, Polymerase Chain Reaction, Biochemistry, Exon, Endocrinology, Thyrotropin-releasing hormone receptor, Cricetinae, Internal medicine, Gene expression, medicine, Animals, Humans, Pituitary Neoplasms, Amino Acid Sequence, RNA, Messenger, Aged, Aged, 80 and over, Messenger RNA, Base Sequence, Receptors, Thyrotropin-Releasing Hormone, Biochemistry (medical), Alternative splicing, Pituitary tumors, Middle Aged, medicine.disease, Peptide Fragments, medicine.anatomical_structure, Pituitary Gland, Female |
| الوصف: | We measured the amounts of TRH receptor (TRHR) messenger ribonucleic acid (mRNA) in human normal pituitary and pituitary tumors and found a novel transcript of the TRHR gene. Competitive PCR revealed expression of the TRHR mRNA in all pituitary adenomas examined, and its level was variable and similar to that in the normal pituitary. When the C-terminal region was amplified by PCR, an additional short product was observed. Cloning and sequence analysis of this short fragment revealed that the deleted sequence corresponded exactly to the 5'-sequence of exon 3, indicating alternative splicing of the TRHR mRNA. This alternative splicing resulted in a frame shift, yielding a C-terminal truncated protein (HTRHR2) on translation. Expression analysis of HTRHR2 in Chinese hamster ovary cells showed no significant binding to [3HIMeTRH or response of intracellular calcium to TRH administration. However, the mRNA ratio of HTRHR2 vs. the wild type (HTRHR1) was significantly different among pituitary tumors. The highest ratio was observed in prolactinomas (30%), and almost no detectable expression was found in GH-producing tumors. These findings indicate that this novel transcript of the human TRH receptor gene is produced in a tumor-specific manner and may be a useful parameter for evaluation of individual pituitary tumors. |
| تدمد: | 1945-7197 0021-972X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb6d6512347c9d75788ebc2bc0c55815 https://doi.org/10.1210/jcem.82.12.4438 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....fb6d6512347c9d75788ebc2bc0c55815 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19457197 0021972X |
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