The Roles of 4β-Hydroxywithanolide E from Physalis peruviana on the Nrf2-Anti-Oxidant System and the Cell Cycle in Breast Cancer Cells
| العنوان: | The Roles of 4β-Hydroxywithanolide E from Physalis peruviana on the Nrf2-Anti-Oxidant System and the Cell Cycle in Breast Cancer Cells |
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| المؤلفون: | Yang Chang Wu, Bang Jau You, Fei Ching Chang, Te-Ling Lu, Chia Lin Lee, Chieh Yu Peng, Hong-Zin Lee, Wen-Hsin Lin |
| المصدر: | The American Journal of Chinese Medicine. 44:617-636 |
| بيانات النشر: | World Scientific Pub Co Pte Lt, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, MAPK/ERK pathway, Programmed cell death, Physalis, NF-E2-Related Factor 2, Apoptosis, Breast Neoplasms, Ataxia Telangiectasia Mutated Proteins, Biology, Antioxidants, Superoxide dismutase, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Animals, Humans, Withanolides, chemistry.chemical_classification, Reactive oxygen species, Cell Cycle, General Medicine, Cell cycle, Antineoplastic Agents, Phytogenic, Glutathione, Cell biology, Disease Models, Animal, 030104 developmental biology, Complementary and alternative medicine, chemistry, 030220 oncology & carcinogenesis, MCF-7 Cells, biology.protein, Signal transduction, Reactive Oxygen Species, DNA Damage, Phytotherapy, Signal Transduction |
| الوصف: | 4[Formula: see text]-Hydroxywithanolide E is an active component of the extract of Physalis peruviana that has been reported to exhibit antitumor effects. Although the involvement of reactive oxygen species (ROS) production and the ataxia-telangiectasia mutated protein (ATM)-dependent DNA damage signaling pathway in 4[Formula: see text]-hydroxywithanolide E-induced apoptosis of breast cancer MCF-7 cells was demonstrated in our previous study, the relationship between ROS production and the cellular defense system response in 4[Formula: see text]-hydroxywithanolide E-induced cell death requires further verification. The present study suggests that ROS play an important role in 4[Formula: see text]-hydroxywithanolide E-induced MCF-7 cell death in which anti-oxidants, such as glutathione or N-acetylcysteine, can resist the 4[Formula: see text]-hydroxywithanolide E-induced accumulation of ROS and cell death. Furthermore, N-acetylcysteine or glutathione can reverse the 4[Formula: see text]-hydroxywithanolide E-induced changes in the cell cycle distribution and the expression of cell cycle regulators. We found that the 4[Formula: see text]-hydroxywithanolide E-induced ROS accumulation was correlated with the upregulation of Nrf2 and Nrf2-downstream genes, such as antioxidative defense enzymes. In general, the activity of Nrf2 is regulated by the Ras signalling pathway. However, we demonstrated that Nrf2 was activated during 4[Formula: see text]-hydroxywithanolide E-induced MCF-7 cell death in spite of the 4[Formula: see text]-hydroxywithanolide E-induced inhibition of the Ras/Raf/ERK pathway. The activity and protein expression of superoxide dismutase and catalase were involved in the 4[Formula: see text]-hydroxywithanolide E-induced ROS production in MCF-7 cells. Furthermore, 4[Formula: see text]-hydroxywithanolide E was demonstrated to significantly reduce the sizes of the tumor nodules in the human breast cancer MDA-MB231 xenograft tumor model. |
| تدمد: | 1793-6853 0192-415X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb8ffda4bb695e61b25046ed758f1ce6 https://doi.org/10.1142/s0192415x16500348 |
| رقم الأكسشن: | edsair.doi.dedup.....fb8ffda4bb695e61b25046ed758f1ce6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17936853 0192415X |
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