PddCas: A Polydisperse Droplet Digital CRISPR/Cas-Based Assay for the Rapid and Ultrasensitive Amplification-Free Detection of Viral DNA/RNA
| العنوان: | PddCas: A Polydisperse Droplet Digital CRISPR/Cas-Based Assay for the Rapid and Ultrasensitive Amplification-Free Detection of Viral DNA/RNA |
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| المؤلفون: | Yingying Xue, Xinyi Luo, Wenfei Xu, Ke Wang, Mengqi Wu, Lei Chen, Gewei Yang, Kun Ma, Ming Yao, Qinghe Zhou, Qingshan Lv, Xuhui Li, Jianhua Zhou, Jiasi Wang |
| المصدر: | Analytical Chemistry. |
| بيانات النشر: | American Chemical Society (ACS), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Analytical Chemistry |
| الوصف: | Clustered regularly interspaced short palindromic repeats (CRISPR)-based assays have been an emerging diagnostic technology for pathogen diagnosis. In this work, we developed a polydisperse droplet digital CRISPR-Cas-based assay (PddCas) for the rapid and ultrasensitive amplification-free detection of viral DNA/RNA with minimum instruments. LbaCas12a and LbuCas13a were used for the direct detection of viral DNA and RNA, respectively. The reaction mixtures were partitioned with a common vortex mixer to generate picoliter-scale polydisperse droplets in several seconds. The limit of detection (LoD) for the target DNA and RNA is approximately 100 aM and 10 aM, respectively, which is about 3 × 10 |
| تدمد: | 1520-6882 0003-2700 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc0f39309ee2c0f561b6dbba9f5ec429 https://doi.org/10.1021/acs.analchem.2c03590 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....fc0f39309ee2c0f561b6dbba9f5ec429 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15206882 00032700 |
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