Effect of Drought Stress during Soybean R2–R6 Growth Stages on Sucrose Metabolism in Leaf and Seed
| العنوان: | Effect of Drought Stress during Soybean R2–R6 Growth Stages on Sucrose Metabolism in Leaf and Seed |
|---|---|
| المؤلفون: | Yanli Du, Qiang Zhao, Futi Xie, Huijun Zhang, Liru Chen, Jun-Jiang Wu, Xingdong Yao |
| المصدر: | International Journal of Molecular Sciences Volume 21 Issue 2 International Journal of Molecular Sciences, Vol 21, Iss 2, p 618 (2020) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Sucrose, Glycine max, Starch, sucrose metabolism, drought, Photosynthesis, 01 natural sciences, Article, Catalysis, lcsh:Chemistry, Inorganic Chemistry, 03 medical and health sciences, chemistry.chemical_compound, Stress, Physiological, sucrose transporter, Physical and Theoretical Chemistry, Sugar, lcsh:QH301-705.5, Molecular Biology, Spectroscopy, biology, Organic Chemistry, fungi, food and beverages, Fructose, General Medicine, source–sink relationship, Droughts, Computer Science Applications, Plant Leaves, Horticulture, 030104 developmental biology, Invertase, lcsh:Biology (General), lcsh:QD1-999, chemistry, Seeds, biology.protein, Sucrose synthase, Sucrose-phosphate synthase, Soybeans, 010606 plant biology & botany |
| الوصف: | Sucrose is the main photosynthesis product of plants and the fundamental carbon skeleton monomer and energy supply for seed formation and development. Drought stress induces decreased photosynthetic carbon assimilation capacity, and seriously affects seed weight in soybean. However, little is known about the relationship between decreases in soybean seed yield and disruption of sucrose metabolism and transport balance in leaves and seeds during the reproductive stages of crop growth. Three soybean cultivars with similar growth periods, &ldquo Shennong17&rdquo &ldquo Shennong8&rdquo and &ldquo Shennong12&rdquo were subjected to drought stress during reproductive growth for 45 days. Drought stress significantly reduced leaf photosynthetic rate, shoot biomass, and seed weight by 63.93, 33.53, and 41.65%, respectively. Drought stress increased soluble sugar contents, the activities of sucrose phosphate synthase, sucrose synthase, and acid invertase enzymes, and up-regulated the expression levels of GmSPS1, GmSuSy2, and GmA-INV, but decreased starch content by 15.13% in leaves. Drought stress decreased the contents of starch, fructose, and glucose in seeds during the late seed filling stages, while it induced sucrose accumulation, which resulted in a decreased hexose-to-sucrose ratio. In developing seeds, the activities of sucrose synthesis and degradation enzymes, the expression levels of genes related to metabolism, and the expression levels of sucrose transporter genes were enhanced during early seed development under drought stress however, under prolonged drought stress, all of them decreased. These results demonstrated that drought stress enhances the capacity for unloading sucrose into seeds and activated sucrose metabolism during early seed development. At the middle and late seed filling stages, sucrose flow from leaves to seeds was diminished, and the balance of sucrose metabolism was impaired in seeds, resulting in seed mass reduction. The different regulation strategies in sucrose allocation, metabolism, and transport during different seed development stages may be one of the physiological mechanisms for soybean plants to resist drought stress. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| DOI: | 10.3390/ijms21020618 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc14810c72ba9b2a121d97ed231465c9 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....fc14810c72ba9b2a121d97ed231465c9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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| DOI: | 10.3390/ijms21020618 |