The LIM homeobox protein mLIM3/Lhx3 induces expression of the prolactin gene by a Pit-1/GHF-1-independent pathway in corticotroph AtT20 cells
| العنوان: | The LIM homeobox protein mLIM3/Lhx3 induces expression of the prolactin gene by a Pit-1/GHF-1-independent pathway in corticotroph AtT20 cells |
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| المؤلفون: | Michel Chrétien, Jean-Christophe Barale, Suzanne Benjannet, Nabil G. Seidah, Stephen E Girardin |
| المساهمون: | Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Molecular Neuroendocrinology, Parasitologie Expérimentale, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | FEBS Letters FEBS Letters, 1998, 431 (3), pp.333-8. ⟨10.1016/S0014-5793(98)00787-X⟩ FEBS Letters, Wiley, 1998, 431 (3), pp.333-8. ⟨10.1016/S0014-5793(98)00787-X⟩ |
| بيانات النشر: | HAL CCSD, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | Cellular differentiation, MESH: Base Sequence, Biochemistry, 0302 clinical medicine, Structural Biology, MESH: Pituitary Gland, mLIM3, Gene expression, MESH: Animals, MESH: Nerve Tissue Proteins, Promoter Regions, Genetic, Regulation of gene expression, 0303 health sciences, MESH: DNA, Cell Differentiation, MESH: Transcription Factors, MESH: Gene Expression Regulation, DNA-Binding Proteins, MESH: Cattle, MESH: Promoter Regions (Genetics), Pituitary Gland, LHX3, Transcription Factor Pit-1, hormones, hormone substitutes, and hormone antagonists, MESH: Cell Differentiation, AtT20 cells, endocrine system, MESH: Rats, LIM-Homeodomain Proteins, Biophysics, MESH: Prolactin, Nerve Tissue Proteins, Biology, Cell Line, Prolactin cell, 03 medical and health sciences, Consensus Sequence, MESH: Homeodomain Proteins, Genetics, Animals, Humans, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, MESH: Consensus Sequence, Molecular Biology, Transcription factor, 030304 developmental biology, Homeodomain Proteins, Messenger RNA, MESH: Humans, Base Sequence, Promoter, Cell Biology, DNA, Molecular biology, Prolactin, Rats, MESH: Cell Line, Pituitary, Gene Expression Regulation, Cattle, MESH: Transcription Factor Pit-1, 030217 neurology & neurosurgery, MESH: DNA-Binding Proteins, Transcription Factors |
| الوصف: | International audience; mLIM3, a member of the LIM homeobox family, was recently demonstrated to be critical for proliferation and differentiation of the pituitary cell lineage. Using a pool of degenerate oligonucleotides we determined the DNA sequence ANNAGGAAA(T/C)GA(CIG)AA as the set preferentially recognized by mLIM3. A nearly identical sequence is found in the prolactin (PRL) promoter, within a 15-mer stretch from nucleotides (nts) -218 to -204 which is highly conserved between human, rat, and bovine. In order to test the hypothesis of a transcriptional effect of mLIM3 on the prolactin promoter, stable transfectants of mLIM3 cDNA in AtT20 tumor cells revealed that PRL mRNA expression was induced in 3 separate stable clones. Gel retardation experiments performed using nuclear extracts isolated from one of the AtT20/mLIM3 stable transfectants revealed affinity towards the 15-mer element of the PRL promoter. From these results, we propose that the PRL promoter element (nts -218 to -204) could be functional in vivo. Finally, we demonstrate that in AtT20 cells prolactin mRNA expression is not induced by the Pit-1/GHF-1 pathway and that growth hormone mRNA is not detected concomitantly with prolactin. We conclude that mLIM3 may play a key role in inducing PRL gene expression in lactotrophs by binding to a conserved motif close to a Pit-1/GHF-1 site within the proximal PRL promoter. |
| اللغة: | English |
| تدمد: | 0014-5793 1873-3468 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc17e4103c8fd7032b3f32a6fedaf5ff https://hal.science/hal-00126414 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....fc17e4103c8fd7032b3f32a6fedaf5ff |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00145793 18733468 |
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