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Effect of inorganic and organic bioactive signals decoration on the biological performance of chitosan scaffolds for bone tissue engineering

التفاصيل البيبلوغرافية
العنوان: Effect of inorganic and organic bioactive signals decoration on the biological performance of chitosan scaffolds for bone tissue engineering
المؤلفون: Christian Demitri, Antonella Giuri, Alessandra Soriente, Luigi Ambrosio, Alessandro Sannino, Ines Fasolino, Marta Madaghiele, Maria Grazia Raucci
المساهمون: Soriente, Alessandra, Fasolino, Ine, Raucci, Maria Grazia, Demitri, Christian, Madaghiele, Marta, Giuri, Antonella, Sannino, Alessandro, Ambrosio, Luigi
المصدر: Journal of materials science. Materials in medicine 29 (2018). doi:10.1007/s10856-018-6072-2
info:cnr-pdr/source/autori:Soriente, Alessandra; Fasolino, Ines; Raucci, Maria Grazia; Demitri, Christian; Madaghiele, Marta; Giuri, Antonella; Sannino, Alessandro; Ambrosio, Luigi/titolo:Effect of inorganic and organic bioactive signals decoration on the biological performance of chitosan scaffolds for bone tissue engineering/doi:10.1007%2Fs10856-018-6072-2/rivista:Journal of materials science. Materials in medicine/anno:2018/pagina_da:/pagina_a:/intervallo_pagine:/volume:29
سنة النشر: 2017
مصطلحات موضوعية: Scaffold, Bone Regeneration, Peptide, 02 engineering and technology, Bone tissue, Chitosan, chemistry.chemical_compound, Tissue Scaffold, Coated Materials, Biocompatible, Osteogenesis, Organic Chemicals, Cells, Cultured, chemistry.chemical_classification, biology, Tissue Scaffolds, Osteogenesi, hydroxyapatite, Cell Differentiation, 021001 nanoscience & nanotechnology, Mesenchymal Stem Cell, medicine.anatomical_structure, Osteocalcin, Organic Chemical, 0210 nano-technology, Bone and Bone, Human, Materials science, 0206 medical engineering, Biomedical Engineering, Biophysics, Bioengineering, Bone morphogenetic protein 2, Bone and Bones, Biomaterials, Calcification, Physiologic, Inorganic Chemical, medicine, Animals, Humans, BMP-2 peptide, Tissue Engineering, Animal, Regeneration (biology), Mesenchymal Stem Cells, Biomaterial, 020601 biomedical engineering, Biophysic, chemistry, Cell culture, Inorganic Chemicals, biology.protein
الوصف: The present work is focused on the design of a bioactive chitosan-based scaffold functionalized with organic and inorganic signals to provide the biochemical cues for promoting stem cell osteogenic commitment. The first approach is based on the use of a sequence of 20 amino acids corresponding to a 68-87 sequence in knuckle epitope of BMP-2 that was coupled covalently to the carboxyl group of chitosan scaffold. Meanwhile, the second approach is based on the biomimetic treatment, which allows the formation of hydroxyapatite nuclei on the scaffold surface. Both scaffolds bioactivated with organic and inorganic signals induce higher expression of an early marker of osteogenic differentiation (ALP) than the neat scaffolds after 3 days of cell culture. However, scaffolds decorated with BMP-mimicking peptide show higher values of ALP than the biomineralized one. Nevertheless, the biomineralized scaffolds showed better cellular behaviour than neat scaffolds, demonstrating the good effect of hydroxyapatite deposits on hMSC osteogenic differentiation. At long incubation time no significant difference among the biomineralized and BMP-activated scaffolds was observed. Furthermore, the highest level of Osteocalcin expression (OCN) was observed for scaffold with BMP2 mimic-peptide at day 21. The overall results showed that the presence of bioactive signals on the scaffold surface allows an osteoinductive effect on hMSC in a basal medium, making the modified chitosan scaffolds a promising candidate for bone tissue regeneration.
وصف الملف: STAMPA
تدمد: 1573-4838
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc348acfd982e08f0ef25a516ef8fbfc
https://pubmed.ncbi.nlm.nih.gov/29736686
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....fc348acfd982e08f0ef25a516ef8fbfc
قاعدة البيانات: OpenAIRE
الوصف
تدمد:15734838