Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease
| العنوان: | Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease |
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| المؤلفون: | Vishnu Chintalgattu, Linda L. Lee, Aarif Y. Khakoo |
| المصدر: | FEBS Open Bio |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, CD31, perivascular cells, cardiac pericyte, Primary Cell Culture, Population, Myocardial Infarction, heart, pericytes, General Biochemistry, Genetics and Molecular Biology, Proinflammatory cytokine, Capillary Permeability, Mice, 03 medical and health sciences, 0302 clinical medicine, Coronary Circulation, Animals, Humans, Secretion, education, Research Articles, Cells, Cultured, education.field_of_study, Chemistry, Myocardium, Endothelial Cells, vascular biology, Hypoxia-Inducible Factor 1, alpha Subunit, Myocardial Contraction, Cell Hypoxia, humanities, Cell biology, Disease Models, Animal, 030104 developmental biology, Apoptosis, 030220 oncology & carcinogenesis, cardiovascular system, No-Reflow Phenomenon, CD146, Endothelium, Vascular, Homeostasis, Research Article, Lipoprotein |
| الوصف: | Pericytes (PCs) in general are known to help maintain vessel integrity; however, their specific function in the heart is not well defined. Our in vitro study details NG2+ PDGFRβ+ CD146+ CD34− CD31− CD45− cardiac PC phenotypic and functional characteristics, and their role in vasoregulation in physiological and disease prone environments. Cardiac PCs can potentially regulate blood flow in the heart. Pericytes (PCs)—mural cells that envelop endothelial cells (ECs) of microvessels—regulate tissue‐specific vasculature development as well as maturation and maintenance of endothelial barrier integrity. However, little is known about their tissue‐specific function in the heart. Specifically, the mechanism by which cardiac PCs constrict coronary capillaries remains undetermined. To gain insights into the function of cardiac PCs at the cellular level, we isolated NG2+ PDGFRβ+ CD146+ CD34− CD31− CD45− PCs for detailed characterization. Functionally, we provide evidence that these PCs increased transepithelial electrical resistance and decreased endothelial permeability. We show for the first time that this population of PCs express contractile proteins, are stimulated by adrenergic signaling, and demonstrate stereotypical contraction and relaxation. Furthermore, we also studied for the first time, the PCs in in vitro models of disease. PCs in hypoxia activated the hypoxia‐inducible factor 1 alpha pathway, increased secretion of angiogenic factors, and caused cellular apoptosis. Supraphysiological levels of low‐density lipoprotein decreased PC proliferation and induced lipid droplet accumulation. Elevated glucose levels triggered a proinflammatory response. Taken together, our study characterizes cardiac PCs under in vitro disease conditions and supports the hypothesis that cardiac PCs are key vasoactive cells that can regulate blood flow in the heart. |
| تدمد: | 2211-5463 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd485d8b49a702841936c039051b38ff https://doi.org/10.1002/2211-5463.13021 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....fd485d8b49a702841936c039051b38ff |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22115463 |
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