Fermented Ginseng Contains an Agonist of Peroxisome Proliferator Activated Receptors α and γ
| العنوان: | Fermented Ginseng Contains an Agonist of Peroxisome Proliferator Activated Receptors α and γ |
|---|---|
| المؤلفون: | Toshitsugu Miyazaki, Hisatomi Ito, Yoshiki Kashiwada, Kentaro Igami, Fusako Nakano, Yosuke Shimojo |
| المصدر: | Journal of Medicinal Food. 19:817-822 |
| بيانات النشر: | Mary Ann Liebert Inc, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Agonist, Sapogenins, Ginsenosides, Transcription, Genetic, medicine.drug_class, Panax, Medicine (miscellaneous), Peroxisome proliferator-activated receptor, Biology, Cell Line, 03 medical and health sciences, Ginseng, 0302 clinical medicine, Transcription (biology), Chlorocebus aethiops, medicine, Animals, PPAR alpha, Rats, Wistar, Receptor, Transcription factor, chemistry.chemical_classification, Nutrition and Dietetics, Molecular Structure, Plant Extracts, food and beverages, Lipid metabolism, Lacticaseibacillus paracasei, Lipid Metabolism, PPAR gamma, Glucose, 030104 developmental biology, chemistry, Nuclear receptor, Biochemistry, 030220 oncology & carcinogenesis, Fermentation, Stearic Acids |
| الوصف: | Peroxisome proliferator activated receptor (PPAR) is a nuclear receptor that is one of the transcription factors regulating lipid and glucose metabolism. Fermented ginseng (FG) is a ginseng fermented by Lactobacillus paracasei A221 containing minor ginsenosides and metabolites of fermentation. DNA microarray analysis of rat liver treated with FG indicated that FG affects on lipid metabolism are mediated by PPAR-α. To identify a PPAR-α agonist in FG, PPAR-α transcription reporter assay-guided fractionation was performed. The fraction obtained from the MeOH extract of FG, which showed potent transcription activity of PPAR-α, was fractionated by silica gel column chromatography into 16 subfractions, and further separation and crystallization gave compound 1 together with four known constituents of ginseng, including 20(R)- and 20(S)-protopanaxadiol, and 20(R)- and 20(S)-ginsenoside Rh1. The structure of compound 1 was identified as 10-hydroxy-octadecanoic acid by (1)H- and (13)C-NMR spectra and by EI-MS analysis of the methyl ester of 1. Compound 1 demonstrated much higher transcription activity of PPAR-α than the other isolated compounds. In addition, compound 1 also showed 5.5-fold higher transcription activity of PPAR-γ than vehicle at the dose of 20 μg/mL. In the present study, we identified 10-hydroxy-octadecanoic acid as a dual PPAR-α/γ agonist in FG. Our study suggested that metabolites of fermentation, in addition to ginsenosides, contribute to the health benefits of FG. |
| تدمد: | 1557-7600 1096-620X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fdad0cfc1fcb3b4c8f6216697ad9c832 https://doi.org/10.1089/jmf.2016.3673 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....fdad0cfc1fcb3b4c8f6216697ad9c832 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15577600 1096620X |
|---|