Chemotactic Responses of Jurkat Cells in Microfluidic Flow-Free Gradient Chambers
| العنوان: | Chemotactic Responses of Jurkat Cells in Microfluidic Flow-Free Gradient Chambers |
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| المؤلفون: | Fatima N. Syed-Picard, Lance A. Davidson, Adam Wood, Philip R. LeDuc, Pawel Kalinski, Kyle Justus, Utku M. Sonmez, Weijian Jiang |
| المصدر: | Micromachines Volume 11 Issue 4 Micromachines, Vol 11, Iss 384, p 384 (2020) |
| بيانات النشر: | MDPI, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Chemokine, lcsh:Mechanical engineering and machinery, concentration gradient, microfluidics, Motility, Context (language use), 02 engineering and technology, Jurkat cells, Article, Extracellular matrix, 03 medical and health sciences, Live cell imaging, Extracellular, lcsh:TJ1-1570, Electrical and Electronic Engineering, chemotaxis, microfabrication, 030304 developmental biology, 0303 health sciences, biology, Chemistry, Mechanical Engineering, Chemotaxis, 021001 nanoscience & nanotechnology, Control and Systems Engineering, Biophysics, biology.protein, 0210 nano-technology |
| الوصف: | Gradients of soluble molecules coordinate cellular communication in a diverse range of multicellular systems. Chemokine-driven chemotaxis is a key orchestrator of cell movement during organ development, immune response and cancer progression. Chemotaxis assays capable of examining cell responses to different chemokines in the context of various extracellular matrices will be crucial to characterize directed cell motion in conditions which mimic whole tissue conditions. Here, a microfluidic device which can generate different chemokine patterns in flow-free gradient chambers while controlling surface extracellular matrix (ECM) to study chemotaxis either at the population level or at the single cell level with high resolution imaging is presented. The device is produced by combining additive manufacturing (AM) and soft lithography. Generation of concentration gradients in the device were simulated and experimentally validated. Then, stable gradients were applied to modulate chemotaxis and chemokinetic response of Jurkat cells as a model for T lymphocyte motility. Live imaging of the gradient chambers allowed to track and quantify Jurkat cell migration patterns. Using this system, it has been found that the strength of the chemotactic response of Jurkat cells to CXCL12 gradient was reduced by increasing surface fibronectin in a dose-dependent manner. The chemotaxis of the Jurkat cells was also found to be governed not only by the CXCL12 gradient but also by the average CXCL12 concentration. Distinct migratory behaviors in response to chemokine gradients in different contexts may be physiologically relevant for shaping the host immune response and may serve to optimize the targeting and accumulation of immune cells to the inflammation site. Our approach demonstrates the feasibility of using a flow-free gradient chamber for evaluating cross-regulation of cell motility by multiple factors in different biologic processes. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2072-666X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fdb76b49af573f0e6ac929ce0f8ce603 http://europepmc.org/articles/PMC7231302 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....fdb76b49af573f0e6ac929ce0f8ce603 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2072666X |
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