Bacterial diversity in organic and conventional Minas Frescal cheese production using targeted 16S rRNA sequencing
| العنوان: | Bacterial diversity in organic and conventional Minas Frescal cheese production using targeted 16S rRNA sequencing |
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| المؤلفون: | Liliana O. Rocha, Giovana Rueda Barboza, Marcelo Falsarella Carazzolle, Nathália Cristina Cirone Silva, Anderson Clayton da Silva Abreu, Vera Lúcia Mores Rall, Bruna Lourenço Crippa |
| المساهمون: | Universidade Estadual de Campinas (UNICAMP), Universidade Estadual Paulista (UNESP) |
| المصدر: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | biology, business.industry, Moraxellaceae, 16S ribosomal RNA, biology.organism_classification, Applied Microbiology and Biotechnology, Enterobacteriaceae, DNA sequencing, Biotechnology, business, Planococcaceae, Gene, Bacteria, Food Science, Molecular identification |
| الوصف: | Made available in DSpace on 2022-04-28T19:41:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-11-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Centro Nacional de Pesquisa em Energia e Materiais Ministério da Ciência, Tecnologia, Inovações e Comunicações Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Molecular identification and the study of bacteria in processed food products can be challenging or suffer limitations when using conventional methods. However, with Next Generation Sequencing (NGS), one can identify bacterial species with a significantly higher detection sensitivity using a 16S rRNA target gene. This study aimed to characterise the bacterial diversity of the organic and conventional production of Minas Frescal cheese, through the NGS analysis of the 16S rRNA gene. DNA was extracted from 96 samples, 48 from organic production and 48 from conventional production. The bacterial families and genera with the highest prevalence were identified. The most prevalent families were Enterobacteriaceae, Planococcaceae and Moraxellaceae. The NGS 16S rRNA tool proved to be very useful for bacterial characterisation, but it is still not possible to say whether a product contains more or less bacteria based on organic production, as there are several factors that contribute to contamination. Department of Food Science School of Food Engineering (FEA) University of Campinas Department of Genetics and Molecular Biology Institute of Biology (IB) University of Campinas Department of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP) Department of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP) FAPESP: 2016/02118-7 FAPESP: 2018/00986-7 |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fdd8630afe4a600cd883ecd64def7aac |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....fdd8630afe4a600cd883ecd64def7aac |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |