ALIX is recruited temporarily into HIV-1 budding sites at the end of gag assembly
| العنوان: | ALIX is recruited temporarily into HIV-1 budding sites at the end of gag assembly |
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| المؤلفون: | Mourad Bendjennat, Jeff Ballew, Michael B. Landesman, Pei-I Ku, Saveez Saffarian |
| المصدر: | PLoS ONE PLoS ONE, Vol 9, Iss 5, p e96950 (2014) |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Vacuolar Proton-Translocating ATPases, Endosome, viruses, Biophysics, lcsh:Medicine, Cell Cycle Proteins, macromolecular substances, Biology, gag Gene Products, Human Immunodeficiency Virus, Microbiology, ESCRT, Virus, 03 medical and health sciences, Membrane fission, Virology, Viruslike Particles, TSG101, Humans, lcsh:Science, 030304 developmental biology, 0303 health sciences, Budding, Multidisciplinary, Microscopy, Confocal, Endosomal Sorting Complexes Required for Transport, Virus Assembly, Physics, 030302 biochemistry & molecular biology, lcsh:R, Calcium-Binding Proteins, Biology and Life Sciences, Group-specific antigen, Viral Replication, 3. Good health, Cell biology, DNA-Binding Proteins, HEK293 Cells, Membrane protein, Physical Sciences, HIV-1, ATPases Associated with Diverse Cellular Activities, lcsh:Q, HeLa Cells, Transcription Factors, Research Article |
| الوصف: | Polymerization of Gag on the inner leaflet of the plasma membrane drives the assembly of Human Immunodeficiency Virus 1 (HIV-1). Gag recruits components of the endosomal sorting complexes required for transport (ESCRT) to facilitate membrane fission and virion release. ESCRT assembly is initiated by recruitment of ALIX and TSG101/ESCRT-I, which bind directly to the viral Gag protein and then recruit the downstream ESCRT-III and VPS4 factors to complete the budding process. In contrast to previous models, we show that ALIX is recruited transiently at the end of Gag assembly, and that most ALIX molecules are recycled into the cytosol as the virus buds, although a subset remains within the virion. Our experiments imply that ALIX is recruited to the neck of the assembling virion and is mostly recycled after virion release. |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fdeab1b9a7d2d39bc6322ec6aedad837 https://pubmed.ncbi.nlm.nih.gov/24834918 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....fdeab1b9a7d2d39bc6322ec6aedad837 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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