Adenovirus-Mediated Wnt10b Overexpression Induces Hair Follicle Regeneration

التفاصيل البيبلوغرافية
العنوان: Adenovirus-Mediated Wnt10b Overexpression Induces Hair Follicle Regeneration
المؤلفون: Ji-Xing Ye, Tian Yang, Ke Yang, Yuhong Li, Haiying Guo, Kun Zhang, Xiaohua Lian, Fang Deng, Yizhan Xing
المصدر: Journal of Investigative Dermatology. 133:42-48
بيانات النشر: Elsevier BV, 2013.
سنة النشر: 2013
مصطلحات موضوعية: Gene Expression Regulation, Viral, medicine.medical_specialty, Small interfering RNA, Genetic Vectors, Active Transport, Cell Nucleus, Dermatology, Biochemistry, Adenoviridae, Mice, In vivo, Internal medicine, medicine, Animals, Regeneration, Gene Silencing, Intradermal injection, Wnt Signaling Pathway, Molecular Biology, beta Catenin, Gene knockdown, integumentary system, biology, Regeneration (biology), Mouse mammary tumor virus, Cell Biology, Hair follicle, biology.organism_classification, In vitro, Up-Regulation, Cell biology, Mice, Inbred C57BL, Wnt Proteins, medicine.anatomical_structure, Endocrinology, Female, sense organs, Hair Follicle
الوصف: Hair follicles periodically undergo regeneration. The balance between activators and inhibitors may determine the time required for telogen hair follicles to reenter anagen. We previously reported that Wnt10b (wingless-type mouse mammary tumor virus integration site family member 10b) could promote the growth of hair follicles in vitro. To unveil the roles of Wnt10b in hair follicle regeneration, we established an in vivo mouse model using intradermal injection. On the basis of this model, we found that Wnt10b could induce the biological switch of hair follicles from telogen to anagen when overexpressed in the skin. The induced hair follicles expressed structure markers and could cycle normally into catagen. Conversely, anagen onset was abrogated by the knockdown of Wnt10b with small interfering RNA (siRNA). The Wnt10b aberrant expression data suggest that it is one of the activators of hair follicle regeneration. The β-catenin protein is translocated to the nucleus in Wnt10b-induced hair follicles. The biological effects of Wnt10b were abrogated when β-catenin expression was downregulated with siRNA. These data revealed that Wnt10b might induce hair follicle regeneration in vivo via the enhanced activation of the canonical Wnt signaling pathway. To our knowledge, our data provide previously unreported insights into the regulation of hair follicle cycling and provide potential therapeutic targets for hair follicle–related diseases.
تدمد: 0022-202X
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe1e91793a1353dc22967909c4cf0e8f
https://doi.org/10.1038/jid.2012.235
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....fe1e91793a1353dc22967909c4cf0e8f
قاعدة البيانات: OpenAIRE