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Screening of anti-microbial, anti-biofilm activity, and cytotoxicity analysis of a designed polyherbal formulation against shigellosis

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العنوان: Screening of anti-microbial, anti-biofilm activity, and cytotoxicity analysis of a designed polyherbal formulation against shigellosis
المؤلفون: Deepmala Sharma, Devendra Singh, Vishnu Agarwal
المصدر: Journal of Ayurveda and Integrative Medicine, Vol 12, Iss 4, Pp 601-606 (2021)
Journal of Ayurveda and Integrative Medicine
بيانات النشر: Elsevier, 2021.
سنة النشر: 2021
مصطلحات موضوعية: Shigellosis, biology, Chemistry, Biofilm, RZ409.7-999, Brine shrimp, Bacterial growth, Antimicrobial, biology.organism_classification, medicine.disease, Antibiofilm, Microbiology, Shigella flexneri, Complementary and alternative medicine, Formulation, Original Research Article (Experimental), Drug Discovery, Anti-quorum sensing, medicine, Cytotoxicity, Miscellaneous systems and treatments, Chromobacterium violaceum
الوصف: Background: Shigellosis is an infectious intestinal disease common in rural communities. In developing countries, shigellosis is caused predominantly by Shigella flexneri and has been determined as a major cause of morbidity and mortality. Objective: The study investigates the anti-biofilm, anti-microbial, and anti-shigellosis activity of a designed formulation. Materials and methods: The potential of the formulation against S. flexneri (MTCC 1457) was investigated using a well-diffusion assay. Further, the effect of the designed formulation on bacterial growth and biofilm formation was analyzed by the spectrophotometry method. Anti-quorum sensing activity was analyzed using Chromobacterium violaceum CV026, a bacterial strain. Finally, the cytotoxicity of the formulation was examined by using cell line and brine shrimp lethality assay. Results: The MIC value of the aqueous extract of the formulation was 2.4 mg ml−1 and an inhibitory zone of 23 mm was observed. On the other side, the formulation significantly inhibited the bacterial growth, biofilm formation (23.78%), violacein inhibition (27.68%) at 0.6 mg ml−1 concentration (did not significantly affect the growth curves) and was found non-toxic in cell assay and brine shrimp lethality assay. Conclusion: According to the result obtained, the designed formulation was found effective and non-toxic, so it can be used to treat shigellosis and Shigella-related infections.
اللغة: English
تدمد: 0975-9476
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fec13cea0a8888c9d8c65342a46d1e03
http://www.sciencedirect.com/science/article/pii/S0975947621001157
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....fec13cea0a8888c9d8c65342a46d1e03
قاعدة البيانات: OpenAIRE
الوصف
تدمد:09759476