Characterization of Some Bacterial Strains Isolated from Animal Clinical Materials and Identified as Corynebacterium xerosis by Molecular Biological Techniques
| العنوان: | Characterization of Some Bacterial Strains Isolated from Animal Clinical Materials and Identified as Corynebacterium xerosis by Molecular Biological Techniques |
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| المؤلفون: | María V. Latre, Lucía Palacios, Gema Chacón, Ana I. Vela, José F. Fernández-Garayzábal, Kent Molin, Ana Fernández, Enevold Falsen |
| المصدر: | Journal of Clinical Microbiology. 48:3138-3145 |
| بيانات النشر: | American Society for Microbiology, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | DNA, Bacterial, Microbiology (medical), medicine.medical_specialty, Epidemiology, Molecular Sequence Data, Corynebacterium, Biology, DNA, Ribosomal, DNA sequencing, Microbiology, Bacterial Proteins, RNA, Ribosomal, 16S, Molecular genetics, DNA, Ribosomal Spacer, medicine, Animals, Cluster Analysis, Phylogeny, Genetics, Corynebacterium Infections, DNA-Directed RNA Polymerases, Sequence Analysis, DNA, Ribosomal RNA, rpoB, 16S ribosomal RNA, biology.organism_classification, DNA Fingerprinting, Bacterial Typing Techniques, DNA profiling, Restriction fragment length polymorphism, Polymorphism, Restriction Fragment Length |
| الوصف: | Eighteen Corynebacterium xerosis strains isolated from different animal clinical specimens were subjected to phenotypic and molecular genetic studies. On the basis of the results of the biochemical characterization, the strains were tentatively identified as C. xerosis . Phylogenetic analysis based on comparative analysis of the sequences of 16S rRNA and rpoB genes revealed that the 18 strains were highly related to C. xerosis , C. amycolatum , C. freneyi , and C. hansenii . There was a good concordance between 16S rRNA and partial rpoB gene sequencing results, although partial rpoB gene sequencing allowed better differentiation of C. xerosis . Alternatively, C. xerosis was also differentiated from C. freneyi and C. amycolatum by restriction fragment length polymorphism analysis of the 16S-23S rRNA gene intergenic spacer region. Phenotypic characterization indicated that besides acid production from d -turanose and 5-ketogluconate, 90% of the strains were able to reduce nitrate. The absence of the fatty acids C 14:0 , C 15:0 , C 16:1 ϖ7 c , and C 17:1 ϖ8 c can also facilitate the differentiation of C. xerosis from closely related species. The results of the present investigation demonstrated that for reliable identification of C. xerosis strains from clinical samples, a combination of phenotypic and molecular-biology-based identification techniques is necessary. |
| تدمد: | 1098-660X 0095-1137 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fefd09484549fe5a337d222065d8891b https://doi.org/10.1128/jcm.02373-09 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....fefd09484549fe5a337d222065d8891b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1098660X 00951137 |
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