Major role of pKpQIL-like plasmids in the early dissemination of KPC-type carbapenemases in the UK
| العنوان: | Major role of pKpQIL-like plasmids in the early dissemination of KPC-type carbapenemases in the UK |
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| المؤلفون: | Katie L. Hopkins, Michel Doumith, Jacqueline Findlay, Neil Woodford, Andrew Dodgson, H Hirani, David M. Livermore |
| المصدر: | Journal of Antimicrobial Chemotherapy. 72:2241-2248 |
| بيانات النشر: | Oxford University Press (OUP), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Gene Transfer, Horizontal, Klebsiella pneumoniae, 030106 microbiology, medicine.disease_cause, Polymerase Chain Reaction, beta-Lactamases, Microbiology, 03 medical and health sciences, Plasmid, Bacterial Proteins, Enterobacteriaceae, Disease Transmission, Infectious, medicine, Pharmacology (medical), Escherichia coli, Pharmacology, Molecular Epidemiology, biology, Electroporation, Enterobacteriaceae Infections, Outbreak, Sequence Analysis, DNA, Enterobacter, biology.organism_classification, United Kingdom, Infectious Diseases, Multilocus sequence typing, Multilocus Sequence Typing, Plasmids |
| الوصف: | Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae were first seen in the UK in 2003 and have been increasingly reported since 2010, largely owing to an ongoing outbreak in North-West England. We examined the role of clonal spread and plasmid transmission in their emergence.Isolates comprised KPC-positive K. pneumoniae ( n = 33), Escherichia coli ( n = 7) and Enterobacter spp. ( n = 4) referred to the national reference laboratory between 2008 and 2010 from 17 UK centres, including three in North-West England. Isolates were typed by MLST. Plasmids were transferred by electroporation and characterized by PCR or sequencing. PCR screening assays were developed to distinguish plasmid pKpQIL variants.The K. pneumoniae isolates included 10 STs, of which three belonged to clonal group (CG) 258. CG258 ( n = 19) isolates were detected in 13 centres but accounted for only 7/19 (36.8%) of those from North-West England. Most KPC-producers (37/44, 84.1%), including 16/19 CG258 K. pneumoniae , carried bla KPC on IncFII K2 plasmids. Sequencing of a subset of these plasmids ( n = 11) revealed similarities with published pKpQIL. One variant, pKpQIL-UK [identified in K. pneumoniae CG258 ( n = 5) and ST468 ( n = 1) isolates from distinct centres] had only a few nucleotide changes from classical pKpQIL, whereas pKpQIL-D1 ( n = 1) and pKpQIL-D2 ( n = 4), from isolates of various species in the North-West, harboured large variations, reflecting replacement of the partitioning and replication functions and potentially thereby facilitating spread. PCR revealed that 36/37 (97.3%) IncFII K2 -type plasmids in KPC-positive isolates had pKpQIL markers.pKpQIL-like plasmids played a major role in the early dissemination of KPC enzymes in the UK. |
| وصف الملف: | application/pdf |
| تدمد: | 1460-2091 0305-7453 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff0a568a8a664c5a629a8dceba77e666 https://doi.org/10.1093/jac/dkx141 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ff0a568a8a664c5a629a8dceba77e666 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602091 03057453 |
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