European hedgehogs (Erinaceus europaeus) as a natural reservoir of methicillin-resistant Staphylococcus aureus carrying mecC in Denmark
| العنوان: | European hedgehogs (Erinaceus europaeus) as a natural reservoir of methicillin-resistant Staphylococcus aureus carrying mecC in Denmark |
|---|---|
| المؤلفون: | Sophie Lund Rasmussen, Thomas Bjørneboe Berg, Owen R. Jones, Øystein Angen, Jesper Larsen, Rien E. van Wijk, Anders Rhod Larsen |
| المصدر: | Rasmussen, S L, Larsen, J, van Wijk, R E, Jones, O R, Berg, T B, Angen, Ø & Larsen, A R 2019, ' European hedgehogs (Erinaceus europaeus) as a natural reservoir of methicillin-resistant Staphylococcus aureus carrying mecC in Denmark ', PLOS ONE, vol. 14, no. 9, e0222031 . https://doi.org/10.1371/journal.pone.0222031 PLoS ONE, Vol 14, Iss 9, p e0222031 (2019) PLoS ONE |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Veterinary medicine, Meticillin, Staphylococcus, Denmark, Wildlife, medicine.disease_cause, Tryptic soy broth, Geographical locations, chemistry.chemical_compound, Zoonoses, Pathology and laboratory medicine, Mammals, Multidisciplinary, Erinaceus, biology, Eukaryota, Agriculture, Medical microbiology, Habitats, Europe, Infectious Diseases, Staphylococcus aureus, Hedgehogs, Vertebrates, Medicine, Pathogens, medicine.drug, Research Article, Methicillin-Resistant Staphylococcus aureus, Livestock, Animal Types, Science, 030106 microbiology, Microbiology, Recombinases, 03 medical and health sciences, Bacterial Proteins, medicine, Animals, Natural reservoir, European Union, Hedgehog, Ecosystem, Disease Reservoirs, Medicine and health sciences, Models, Statistical, Bacteria, Ecology and Environmental Sciences, Organisms, Biology and Life Sciences, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, bacterial infections and mycoses, Methicillin-resistant Staphylococcus aureus, Microbial pathogens, 030104 developmental biology, Carriage, chemistry, Amniotes, Bacterial pathogens, People and places, Zoology |
| الوصف: | OBJECTIVES: A recent study from Sweden showed that European hedgehogs may constitute a reservoir for methicillin-resistant Staphylococcus aureus (MRSA), but this host-parasite relationship remains to be investigated in other countries. In this study, we therefore sought to: 1) determine the dissemination of MRSA in European hedgehogs throughout Denmark; 2) investigate determinants of MRSA carriage in hedgehogs; 3) determine the potential for zoonotic transmission of MRSA from hedgehogs to humans; and 4) characterise the detected MRSA on both a phenotypic and molecular level.METHODS: Nasal swabs were taken from 188 dead hedgehogs collected by volunteers throughout Denmark to determine the occurrence of MRSA. Additionally, 16 hedgehog rehabilitators were tested for potential zoonotic transmission of MRSA from hedgehogs to humans. The swabs were incubated in tryptic soy broth supplemented with 6.5% NaCl, followed by spread of 10 μl on Brilliance MRSA 2 agar. One presumptive MRSA colony from each plate was subcultured on 5% blood agar. All S. aureus subcultures were verified by a PCR assay detecting mecA, mecC, lukF-PV, scn, and spa, followed by spa typing.RESULTS: A total of 114 (61%) hedgehogs carried mecC-MRSA, whereas none carried mecA-MRSA. The detected mecC-MRSA belonged to two genetic lineages CC130 (spa-types: t528, t843, t1048, t3256, t3570, t6220, t17133) and CC1943 (spa-types: t978, t2345, t3391, t8835, t16868), 52% of which were spa-type t843 (CC130).The detection rate of mecC-MRSA in the hedgehogs was similar regardless of cause of death, sex, region and habitat type. None of the hedgehog rehabilitators carried MRSA.CONCLUSIONS: This nationwide study confirms a high occurrence of mecC-MRSA in hedgehogs, which could serve as a natural reservoir for this specific type of MRSA. Furthermore, our study did not find signs of zoonotic transmission of mecC-MRSA to hedgehog rehabilitators. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff1b0d23945f771e378dbe42936e1deb https://findresearcher.sdu.dk:8443/ws/files/153572809/European_hedgehogs_Erinaceus_europaeus_as_a_natural_reservoir_of_methicillin_resistant_Staphylococcus_aureus_carrying_mecC_in_Denmark.pdf |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ff1b0d23945f771e378dbe42936e1deb |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |