Generation of anti-idiotypic antibodies to detect anti-spacer antibody idiotopes in acute thrombotic thrombocytopenic purpura patients
| العنوان: | Generation of anti-idiotypic antibodies to detect anti-spacer antibody idiotopes in acute thrombotic thrombocytopenic purpura patients |
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| المؤلفون: | Inge Pareyn, Rob Fijnheer, Jan Voorberg, Karen Vanhoorelbeke, Simon F. De Meyer, Marina Biganzoli, Bérangère S. Joly, Flora Peyvandi, Hans Deckmyn, An Sofie Schelpe, Elien Roose, Agnès Veyradier, Ilaria Mancini, Paul Coppo |
| المساهمون: | Experimental Vascular Medicine, Landsteiner Laboratory, ACS - Microcirculation |
| المصدر: | Haematologica Haematologica, 104(6), 1268-1276. Ferrata Storti Foundation |
| بيانات النشر: | Ferrata Storti Foundation (Haematologica), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Thrombotic thrombocytopenic purpura, ADAMTS13 Protein, Enzyme-Linked Immunosorbent Assay, Autoantigens, Severity of Illness Index, MICROANGIOPATHY, VALIDATION, Article, Epitopes, 03 medical and health sciences, 0302 clinical medicine, BINDING, Severity of illness, Animals, Humans, Medicine, Clinical significance, Autoantibodies, Hemostasis, Science & Technology, Purpura, Thrombotic Thrombocytopenic, biology, ANTI-ADAMTS13 ANTIBODIES, business.industry, Autoantibody, Idiotopes, Hematology, medicine.disease, CAPLACIZUMAB, ADAMTS13, ADAMTS13 ACTIVITY, Antibodies, Anti-Idiotypic, 3. Good health, PROGNOSTIC VALUE, UREMIC SYNDROME, Immunoglobulin G, Immunology, Anti-idiotypic antibodies, biology.protein, AUTOANTIBODIES, Disease Susceptibility, Antibody, business, Life Sciences & Biomedicine, MAIN IMMUNOGENIC REGION, Protein Binding, 030215 immunology |
| الوصف: | In autoantibody-mediated autoimmune diseases, autoantibody profiling allows patients to be stratified and links autoantibodies with disease severity and outcome. However, in immune-mediated thrombotic thrombocytopenic purpura (iTTP) patients, stratification according to antibody profiles and their clinical relevance has not been fully explored. We aimed to develop a new type of autoantibody profiling assay for iTTP based on the use of anti-idiotypic antibodies. Anti-idiotypic antibodies against 3 anti-spacer autoantibodies were generated in mice and were used to capture the respective anti-spacer idiotopes from 151 acute iTTP plasma samples. We next deciphered these anti-spacer idiotope profiles in iTTP patients and investigated whether these limited idiotope profiles could be linked with disease severity. We developed 3 anti-idiotypic antibodies that recognized particular idiotopes in the anti-spacer autoantibodies II-1, TTP73 or I-9, that are involved in ADAMTS13 binding; 35%, 24% and 42% of patients were positive for antibodies with the II-1, TTP73 and I-9 idiotopes, respectively. Stratifying patients according to the corresponding 8 anti-spacer idiotope profiles provided a new insight into the anti-spacer II-1, TTP73 and I-9 idiotope profiles in these patients. Finally, these limited idiotope profiles showed no association with disease severity. We successfully developed 3 anti-idiotypic antibodies that allowed us to determine the profiles of the anti-spacer II-1, TTP73 and I-9 idiotopes in iTTP patients. Increasing the number of patients and/or future development of additional anti-idiotypic antibodies against other anti-ADAMTS13 autoantibodies might allow idiotope profiles of clinical, prognostic value to be identified. ispartof: HAEMATOLOGICA vol:104 issue:6 pages:1268-1276 ispartof: location:Italy status: published |
| وصف الملف: | Print-Electronic |
| تدمد: | 1592-8721 0390-6078 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff7e43bc2ccbb40bba421ecb097a1bed https://doi.org/10.3324/haematol.2018.205666 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ff7e43bc2ccbb40bba421ecb097a1bed |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15928721 03906078 |
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