Beyond tethering and the LEM domain: MSCellaneous functions of the inner nuclear membrane Lem2
| العنوان: | Beyond tethering and the LEM domain: MSCellaneous functions of the inner nuclear membrane Lem2 |
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| المؤلفون: | Sigurd Braun, Ramón R. Barrales |
| المساهمون: | Friedrich-Baur-Institute (Germany), German Research Foundation |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| بيانات النشر: | Taylor & Francis, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Nuclear Envelope, Heterochromatin, Perinuclear silencing, Heterochromatic silencing, LEM domain, Biology, Domain (software engineering), 03 medical and health sciences, Protein Domains, medicine, Animals, Humans, Gene silencing, Inner membrane, Gene Silencing, Nuclear membrane, MSC domain, Genetics, Tethering, Extra View, Chromatin tethering, Nuclear Proteins, Cell Biology, Chromatin, Cell biology, Protein Transport, 030104 developmental biology, medicine.anatomical_structure |
| الوصف: | The nuclear envelope plays a pivotal role in the functional organization of chromatin. Various inner nuclear membrane (INM) proteins associate with transcriptionally repressed chromatin, which is often found at the nuclear periphery. A prominent example is the conserved family of LEM (LAP2-Emerin-MAN1) domain proteins that interact with DNA-binding proteins and have been proposed to mediate tethering of chromatin to the nuclear membrane. We recently reported that the fission yeast protein Lem2, a homolog of metazoan LEM proteins, contributes to perinuclear localization and silencing of heterochromatin. We demonstrate that binding and tethering of centromeric chromatin depends on the LEM domain of Lem2. Unexpectedly, this domain is dispensable for heterochromatin silencing, which is instead mediated by a different structural domain of Lem2, the MSC (MAN1-Src1 C-terminal) domain. Hence, silencing and tethering by Lem2 can be mechanistically separated. Notably, the MSC domain has multiple functions beyond heterochromatic silencing. Here we discuss the implications of these novel findings for the understanding of this conserved INM protein. This work was supported by grants to S.B. by the European Union Network of Excellence EpiGeneSys (HEALTH-2010-257082), the German Research Foundation (BR 3511/3-1), and the Friedrich-Baur Stiftung. |
| تدمد: | 1949-1042 1949-1034 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff8f3224ff4f2f507c882da127941b14 http://hdl.handle.net/10261/163188 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ff8f3224ff4f2f507c882da127941b14 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 19491042 19491034 |
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