Engineered Escherichia coli strains as platforms for biological production of isoprene
| العنوان: | Engineered Escherichia coli strains as platforms for biological production of isoprene |
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| المؤلفون: | Ju Seok Lee, Hongweon Lee, Hyeok Won Lee, Jung Ho Park, Jin Gyeom Lee, Eun Gyo Lee, Jungoh Ahn, Won-Kyo Kim |
| المصدر: | FEBS Open Bio FEBS Open Bio, Vol 10, Iss 5, Pp 780-788 (2020) |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Microorganism, Protein Engineering, medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, chemistry.chemical_compound, Hemiterpenes, 0302 clinical medicine, scale‐up, Butadienes, Escherichia coli, medicine, Food science, lcsh:QH301-705.5, Research Articles, Isoprene, Chemistry, Genetically engineered, Escherichia coli Proteins, Escherichia coli strains, 030104 developmental biology, lcsh:Biology (General), Productivity (ecology), isoprene production, Biofuels, 030220 oncology & carcinogenesis, Fermentation, bacteria, Research Article |
| الوصف: | Volatile compounds can be produced by fermentation from genetically engineered microorganisms. Escherichia coli strains are mainly used for isoprene production owing to their higher titers; however, this has thus far been confined to only strains BL21, BL21 (DE3), Rosetta, and BW25113. Here, we tested four groups of E. coli strains for improved isoprene production, including K‐12 (DH5α, BW25113, W3110, MG1655, XL1‐Blue, and JM109), B [Rosetta (DE3), BL21, and BL21 (DE3)], Crooks C, and Waksman W strains. The isoprene productivity of BL21 and MG1655 was remarkably higher than that of the others in 5‐L fermentation, and scale‐up fermentation (300 L) of BL21 was successfully performed. This system shows potential for biobased production of fuel and volatile compounds in industrial applications. In this study, we compared 11 engineered recombinant Escherichia coli strains for improved isoprene production using one‐ and two‐vector systems. Our results showed that the isoprene productivity of strains BL21 and MG1655 was markedly higher than that of the other strains in 5‐L fermentation. Moreover, we achieved successful scale‐up fermentation using the BL21 strain in a 300‐L fermentor. |
| تدمد: | 2211-5463 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ffa2729ce02ab14d09af8083869a06b9 https://doi.org/10.1002/2211-5463.12829 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ffa2729ce02ab14d09af8083869a06b9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22115463 |
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