التفاصيل البيبلوغرافية
العنوان:
Apoptosis is Induced in Chlamydia trachomatis-infected HEp-2 Cells by the Addition of a Combination Innate Immune Activation Compounds and the Inhibitor Wedelolactone
المؤلفون:
Huston, WM , Gloeckl, S , de Boer, L , Beagley, KW , Timms, P
سنة النشر:
2011
مصطلحات موضوعية:
Lipopolysaccharides , Microbial Viability , NF-kappa B , Chlamydia trachomatis , Apoptosis , biochemical phenomena, metabolism, and nutrition , Chlamydia Infections , Immunity, Innate , Cell Line , I-kappa B Kinase , Gene Expression Regulation , Coumarins , Interferon Regulatory Factors , Humans , Obstetrics & Reproductive Medicine
الوصف:
Problem Innate immune activation of human cells, for some intracellular pathogens, is advantageous for vacuole morphology and pathogenic viability. It is unknown whether innate immune activation is advantageous to Chlamydia trachomatis viability. Method of study Innate immune activation of HEp-2 cells during Chlamydia infection was conducted using lipopolysaccharide (LPS), polyI:C, and wedelolactone (innate immune inhibitor) to investigate the impact of these conditions on viability of Chlamydia. Results The addition of LPS and polyI:C to stimulate activation of the two distinct innate immune pathways (nuclear factor kappa beta and interferon regulatory factor) had no impact on the viability of Chlamydia. However, when compounds targeting either pathway were added in combination with the specific innate immune inhibitor (wedelolactone) a major impact on Chlamydia viability was observed. This impact was found to be due to the induction of apoptosis of the HEp-2 cells under these conditions. Conclusion This is the first time that induction of apoptosis has been reported in C. trachomatis-infected cells when treated with a combination of innate immune activators and wedelolactone. © 2010 John Wiley and Sons A/S.
وصف الملف:
application/pdf
URL الوصول:
https://explore.openaire.eu/search/publication?articleId=od_______363::2a31ef519f443977ddc791f8fdaa1ff7 https://hdl.handle.net/10453/111505
حقوق:
OPEN
رقم الأكسشن:
edsair.od.......363..2a31ef519f443977ddc791f8fdaa1ff7
قاعدة البيانات:
OpenAIRE
