التفاصيل البيبلوغرافية
| العنوان: |
Attenuation of Liver Pro-Inflammatory Responses by Zingiber officinale via Inhibition of NF-kappa B Activation in High-Fat Diet-Fed Rats |
| المؤلفون: |
Li, XH, McGrath, KCY, Nammi, S, Heather, AK, Roufogalis, BD |
| سنة النشر: |
2012 |
| مصطلحات موضوعية: |
Inflammation, Male, Plant Extracts, NF-kappa B, Anti-Inflammatory Agents, Ginger, Diet, High-Fat, Polymerase Chain Reaction, Rats, Rats, Sprague-Dawley, Liver, Gene Expression Regulation, Hepatocytes, Animals, Humans, Cytokines, Pharmacology & Pharmacy, RNA, Messenger, Cells, Cultured, Signal Transduction |
| الوصف: |
The aim of this study was to investigate whether treatment with a ginger (Zingiber officinale) extract of high-fat diet (HFD)-fed rats suppresses Nuclear factor-kappa B (NF-κB)-driven hepatic inflammation and to subsequently explore the molecular mechanisms in vitro. Adult male Sprague-Dawley rats were treated with an ethanolic extract of Zingiber officinale (400mg/kg) along with a HFD for 6weeks. Hepatic cytokine mRNA levels, cytokine protein levels and NF-κB activation were measured by real-time PCR, Western blot and an NF-κB nuclear translocation assay, respectively. In vitro, cell culture studies were carried out in human hepatocyte (HuH-7) cells by treatment with Zingiber officinale (100μg/mL) for 24hr prior to interleukin-1β (IL-1β, 8ng/mL)-induced inflammation. We showed that Zingiber officinale treatment decreased cytokine gene TNFα and IL-6 expression in HFD-fed rats, which was associated with suppression of NF-κB activation. In vitro, Zingiber officinale treatment decreased NF-κB-target inflammatory gene expression of IL-6, IL-8 and serum amyloid A1 (SAA1), while it suppressed NF-κB activity, IκBα degradation and IκB kinase (IKK) activity. In conclusion, Zingiber officinale suppressed markers of hepatic inflammation in HFD-fed rats, as demonstrated by decreased hepatic cytokine gene expression and decreased NF-κB activation. The study demonstrates that the anti-inflammatory effect of Zingiber officinale occurs at least in part through the NF-κB signalling pathway. © 2011 The Authors. Basic and Clinical Pharmacology and Toxicology © 2011 Nordic Pharmacological Society. |
| وصف الملف: |
application/pdf |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=od_______363::ae3b181e5f47372c26c445c75613d3ac
https://hdl.handle.net/10453/22442 |
| حقوق: |
OPEN |
| رقم الأكسشن: |
edsair.od.......363..ae3b181e5f47372c26c445c75613d3ac |
| قاعدة البيانات: |
OpenAIRE |
