Supkonjunktivalna primjena alogeničnih limbalnih stanica u pasa s poremećajima rožnice
| العنوان: | Supkonjunktivalna primjena alogeničnih limbalnih stanica u pasa s poremećajima rožnice |
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| المؤلفون: | Ana Kovšca Janjatović, Gordan Mršić, Boris Pirkić, Ivana Kiš, Darko Capak, Njetočka Gredelj Šimec, Dubravko Kezić, Daniel Špoljarić, Josip Crnjac, Maja Popović |
| المصدر: | Veterinarski arhiv Volume 85 Issue 5 |
| بيانات النشر: | Faculty of Veterinary Medicine University of Zagreb, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | genetic structures, corneal disorders, allogenic limbal cells, dogs, sense organs, poremećaji rožnice, alogenične limbalne stanice, psi, eye diseases |
| الوصف: | Limbal stem cells play a crucial role in maintaining the integrity of the corneal surface in a healthy condition or after injury through corneal renewal and repair. In pathologic conditions these cells could be replenished with cultivated allogenic limbal cells. In this investigation, limbal stem cells from small fragments of donor tissue were cultivated in vitro for treatment of corneal lesions in dogs. Fourteen dogs were divided into two groups of seven animals each: a group of dogs with corneal lesions treated using the classical veterinary ophthalmology approach, and a group of dogs with corneal lesions treated with the application of cultivated allogenic limbal cells. Regardless of the size and location of the corneal lesions, after 30 days in the group of dogs with corneal lesions treated with the application of cultivated allogenic limbal cells ophthalmological examination showed no signs of any eye disorder except corneal edema (14.29 %). In the group of dogs with corneal lesions cured using the classical veterinary ophthalmology approach, 30 days after the beginning of treatment ophthalmological examination showed 42.86 % mild/incisional blepharospasm, 85.71 % secretion, 100 % observable conjuctival congestion, 14.29 % eye liquor dimming, 85.71 % corneal edema and 85.71 % irritation. After application of cultivated allogenic limbal cells, corneal lesions were completely healed 15 days earlier than the lesions treated with conventional ophthalmological therapy. Allogenic limbal cells were capable of restoring corneal clarity with no development of ocular complications. Limbalne matične stanice igraju ključnu ulogu u održavanju integriteta površine rožnice u zdravom stanju ili nakon ozljede, kroz obnavljanje i popravak rožnice. U patološkim stanjima te se stanice mogu nadomijestiti s uzgojenim alogeničnim limbalnim stanicama. U ovom istraživanju limbalne matične stanice iz malih fragmenata tkiva donora uzgojene su in vitro radi liječenja ozljeda rožnice u pasa. Četrnaest pasa bilo je podijeljeno u dvije skupine od sedam životinja: skupina pasa s ozljedama rožnice liječenim klasičnim veterinarskim oftalmološkim pristupom i skupina pasa s ozljedama rožnice liječenim primjenom in vitro uzgojnih alogeničnih limbalnih stanica. Neovisno o veličini i smještaju lezije rožnice, deset dana nakon primjene stanica, oftalmološki pregled je pokazao 80% manje izražene simptome blefarospazma, 85% smanjenu sekreciju, 43% manje uočljivu konjunktivalnu kongestiju, 31% manji edem rožnice i 55% smanjenu iritaciju u usporedbi s psima liječenim klasičnim oftalmološkim pristupom, bez znakova zamućenja očne vodice ili priraslica. Nakon primjene uzgojeni alogeničnih limbalnih stanica, lezije rožnice u potpunosti su bile izliječene 15 dana ranije nego lezije liječene konvencionalnim oftalmološkim načinom. Supkonjunktivalna primejna limbalnih stanica omogućuje potpunu regeneraciju ozljeda rožnice nakon 30 dana bez obzira na veličinu i opseg defekta. Alogenične limbalne stanice sposobne su obnoviti prozirnost rožnice bez razvoja komplikacija. Iako su postignuti rezultati bili ohrabrujući, potrebna su daljnja istraživanja za optimizaciju protokola liječenja. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1331-8055 0372-5480 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=od_______951::78be566f24f0c2430663209ff0ceab0d https://hrcak.srce.hr/file/218282 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.od.......951..78be566f24f0c2430663209ff0ceab0d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13318055 03725480 |
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