GUT SEROTONIN: REVEALING ITS ROLE IN ANTIMICROBIAL PEPTIDE PRODUCTION

التفاصيل البيبلوغرافية
العنوان: GUT SEROTONIN: REVEALING ITS ROLE IN ANTIMICROBIAL PEPTIDE PRODUCTION
المؤلفون: Kwon, Eric YH
المساهمون: Khan, Waliul I, Medical Sciences
سنة النشر: 2018
مصطلحات موضوعية: Serotonin, Inflammatory Bowel Disease (IBD), digestive system, β-defensin, Peroxisome proliferator-activated receptor gamma (PPAR-γ)
الوصف: Serotonin (5-hydroxytryptamine [5-HT]) is a key enteric signaling molecule that is implicated in many gastrointestinal (GI) disorders, including inflammatory bowel disease (IBD). Enterochromaffin (EC) cells are a key subgroup of enteric endocrine cells and produce the majority of 5-HT via tryptophan hydroxylase 1 (Tph1) in the gut. Recently, we have identified a pivotal role of 5-HT in the pathogenesis of experimental colitis, whereby 5-HT plays as a pro-inflammatory molecule. Gut function as well as pathology rely on interactions with gut microbiota. The intestinal epithelial cells produce antimicrobial peptides (AMPs), maintaining the mucosal barrier by shaping gut microbiota composition. Among the AMPs, β-defensins are the most well investigated subtype in the colon. Aberrant β-defensin expression has been reported in association with various GI disease pathogenesis including IBD. As EC cells are dispersed throughout the intestinal epithelium, it seems possible that 5-HT can modify β-defensin production which can regulate gut inflammation by influencing gut microbial composition. Colitis was induced with dextran sulfate sodium (DSS) in Tph1+/+ and Tph1-/- (which have lower amounts of 5-HT in gut). Tph1-/- mice exhibited higher levels of β-defensin in the colon, compared with wild-type littermates post-DSS. In addition, increased expression of β-defensin in Tph1-/- mice was suppressed by 5-hydroxytryptophan (5-HTP; precursor of 5-HT) treatment. 5-HT treatment resulted in decreased human β-defensin (hBD) 1 and hBD-2 expression in HT-29 cells. Peroxisome proliferator-activated receptor gamma (PPAR-γ) is essential for maintaining β-defensin expression in the colon. GW-9662, PPAR-γ antagonist, reduced mouse β-defensin (mBD) 1 and mBD-3 (orthologue of hBD-2). Furthermore, disrupting 5-HT7 receptors, but not 5-HT3 or 5-HT4, led to enhanced expression of PPAR-γ via ERK1/2-dependent mechanism. These observations provide us with novel information on pivotal role of gut-derived 5-HT in innate immune response and highlight the potential benefits of targeting 5-HT signaling in various GI disorders such as IBD. Thesis Master of Science (MSc)
اللغة: English
URL الوصول: https://explore.openaire.eu/search/publication?articleId=od______1154::9265047c6086810c45061f892ae851e0
http://hdl.handle.net/11375/23397
حقوق: OPEN
رقم الأكسشن: edsair.od......1154..9265047c6086810c45061f892ae851e0
قاعدة البيانات: OpenAIRE