The aim of the thesis was to compare the awareness of employees of the Temelín nuclear power plant and the general public about evacuation in the event of a radiation emergency. For this purpose, a questionnaire survey was carried out. Questionnaires were distributed and filled out using an internet tool for creating questionnaires at survio.com stands. The theoretical part of the work is focused on the Temelín nuclear power plant, its geographical location and technical description. The work is also focused on the radiation safety of nuclear power plants, their workers and residents against radiation. In the theoretical part, selected accidents of the Jaslovské Bohunice, Chernobyl and Fukushima nuclear power plants are further described. For the purposes of the practical part, the same questionnaire was created for the employees of the Temelín nuclear power plant and the general public. The questionnaire is composed of 34 questions. The first 4 questions serve to divide the respondents, and the remaining questions determine the awareness of the respondents about evacuation in the event of a radiation emergency. A total of 100 respondents took part in the survey - 50 employees of the Temelín nuclear power plant (50%) and 50 respondents from the general public (50%). The survey results were evaluated using the internet tool survio.com and the Microsoft Excel software program. The aim of the thesis was to compare the awareness of the general public and employees of the Temelín nuclear power plant about evacuation in the event of a radiation emergency. From the questionnaire survey, it is evident that the level of awareness of the employees of the Temelín nuclear power plant reaches 86%, and the general public only 68%. The result shows that employees are better and more informed than the lay public. Based on this investigation, I would recommend informing the public more about evacuation options in the event of a radiation emergency.