Sveučilište u Zagrebu. Prehrambeno-biotehnološki fakultet. Zavod za prehrambeno-tehnološko inženjerstvo. Laboratorij za tehnologiju vrenja i kvasca., 2021.
Imela je biljka koja se već godinama u narodnoj medicini smatra iznimno zdravom te se koristi protiv mnogih zdravstvenih tegoba. Od imele se pravi poznata Istarska rakija Biska koja se od davnina smatra vrlo ljekovitom. Kako bi se utvrdila antimikrobna aktivnost imele, analizirani su uzorci macerata imele u 25 %, 40 %, 55 % i 70 %-tnom etanolu. Ispitivanje je također provedeno na uzorcima čistog alkohola istog postotka kao i macerati te na četiri komercijalna uzorka Biske. Uzorci su ispitivani na Gram pozitivnim i Gram negativnim bakterijama, bakterijama mliječne kiseline te na kvascima. Svakom mikroorganizmu su određene zone inhibicije za svaki uzorak macerata i Biske te za čistu etanolnu bazu. Najveću zonu inhibicije pokazao je macerat imele u 55 %-tnom etanolu prema Gram negativnoj bakteriji Salmonella thyphimurium. Uzorci Biske su najveći utjecaj pokazali na Gram pozitivnu bakteriju Bacillus subtilis. Mistletoe is a plant to which many health benefits are traditionally attributed, which is why it has been used for years in folk medicine to combat various diseases. In Croatia, it is the main ingredient of the popular Istrian brandy Biska, which is known for its exceptional healing properties. The aim of this study is to determine the antimicrobial activity of mistletoe by analyzing samples obtained by macerating mistletoe in solutions of 25%, 40%, 55% and 70% ethanol. The analysis also included samples of pure alcohol at the same percentage as the macerates and four commercial Biska samples. All samples were analyzed for Gram positive and Gram negative bacteria, lactic acid bacteria and yeasts. Each microorganism had to be observed according to its zones of inhibition in each of the three types of samples - mistletoe macerates, Biska and pure ethanol. Ultimately, the study showed that the largest zone of inhibition belonged to the 55% ethanol macerate against Gram-negative Salmonella typhimurium. In addition, the Biska samples were found to have the most significant effect on Gram positive Bacillus subtilis.
