Water is an important part of the ecosystem for life. With industrialization, pollution in water resources has reached a worrying level. Water pollution due to heavy metals and their increasing concentrations have caused researchers to increase their interest in the subject due to the damage they cause to water ecosystems. It requires serious cost and time to eliminate the pollution caused by heavy metals in water. In recent, years, the use of biosorption method using bacteria to remove heavy metals in water has become widespread. The main reason why this method is preferred is that gram-positive bacteria have a thick peptidoglycan layer on the cell wall and increases the adsorption capacity. In this study. in drinking, waste, river water and artificially prepared samples, batch method of heavy metal biosorption and biosorption competition in multiple prepared heavy metal solutions were investigated. For these processes. Bacillus licheniformth sp. Bacillus subtilis sp. and Bacillus subtilis (ATCC 6051) strains were used as a biosorbent. Biosorbtion of Cd (II), Cu (H), Pb (II), Fe(II), Ni (II) and Zn (II) metals from waters with these biosorbents at different pHs at. 25 degrees C with 0.25 mg LI It was investigated. Surface morphological structures of biosorbents were evaluated using SEM images and element compositions were evaluated using EDAX profile. Element content was determined using ICP-OES. It was found that heavy metal ions were removed up to 98% with maximum biosorption at pH 6.0.